Protein profile

VK055_0063

tRNA (cmo5U34)-methyltransferase

Genome: KpATCC43816

Gene: cmoA AIK78691.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GS19
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Amino acids 247
Annotations 6
Features 14
PDB binders 0
Druggability 0.889

Overview

Basic information about this protein and its source genome.

Accession
VK055_0063
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
cmoA AIK78691.1
Status
annotated
Amino acids
247
Structure source
AlphaFold + ColabFold
EC
2.1.3.-
GO
GO:0002098 The process in which a uridine at position 34 of a tRNA is post-transcriptionally modified. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon. GO:0016743 Catalysis of the transfer of a carboxyl- or carbamoyl group from one compound (donor) to another (acceptor). GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule. GO:1904047 Binding to S-adenosyl-L-methionine. GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
31.855
Localization
Cytoplasmic
ColabFold pLDDT
92.34

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.889
Structure A0A0H3GS19
Pocket Pocket 3
P2Rank 0.964
Structure A0A0H3GS19
Pocket Pocket 1
ColabFold model
FPocket 0.851 · Pocket 1
P2Rank 0.97 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 151 / 4744 genomes with a hit
Normalized 0.032

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0002098 The process in which a uridine at position 34 of a tRNA is post-transcriptionally modified. The wobble nucleoside of the tRNA sequence (position 34) corresponds to the first position of the anticodon.
  • GO:0016743 Catalysis of the transfer of a carboxyl- or carbamoyl group from one compound (donor) to another (acceptor).
  • GO:0008168 Catalysis of the transfer of a methyl group to an acceptor molecule.
  • GO:1904047 Binding to S-adenosyl-L-methionine.
  • GO:0032259 The process in which a methyl group is covalently attached to a molecule.

Sequence Features

Domain/signature hits from InterPro and related databases.

14 records
Show feature table
Start End DB Term Name
18 247 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
18 247 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
4 242 NCBIfam TIGR00740 carboxy-S-adenosyl-L-methionine synthase CmoA
4 242 InterPro IPR005271 Carboxy-S-adenosyl-L-methionine synthase
60 163 CDD cd02440 AdoMet_MTases
3 242 Hamap MF_01589 Carboxy-S-adenosyl-L-methionine synthase [cmoA].
3 242 InterPro IPR005271 Carboxy-S-adenosyl-L-methionine synthase
1 243 PIRSF PIRSF006325 Mtase_YecO
1 243 InterPro IPR005271 Carboxy-S-adenosyl-L-methionine synthase
20 242 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
20 242 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
60 158 Pfam PF13649 Methyltransferase domain
60 158 InterPro IPR041698 Methyltransferase domain 25
5 232 PANTHER PTHR43861 TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GS19
AlphaFold — — full sequence — Viewing
ColabFold VK055_0063
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.889

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 36.25 0.955
2 2.57 0.073
3 1.3 0.014