Protein profile

VK055_0799

PTS system, glucose-like IIB component domain protein

Genome: KpATCC43816

Gene: AIK79422.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GTE9
Export view
Amino acids 510
Annotations 6
Features 55
PDB binders 0
Druggability 0.736

Overview

Basic information about this protein and its source genome.

Accession
VK055_0799
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK79422.1
Status
annotated
Amino acids
510
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
29.961
Localization
CytoplasmicMembrane
ColabFold pLDDT
88.14

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.736
Structure A0A0H3GTE9
Pocket Pocket 21
P2Rank 0.975
Structure A0A0H3GTE9
Pocket Pocket 1
ColabFold model
FPocket 0.851 · Pocket 27
P2Rank 0.985 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 15 / 4744 genomes with a hit
Normalized 0.003

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

55 records
Show feature table
Start End DB Term Name
196 218 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
106 124 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
247 275 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
85 105 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 416 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
1 416 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
306 322 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
191 195 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
170 190 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
446 463 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
446 463 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
371 381 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
345 350 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
125 149 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
74 84 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
431 498 CDD cd00212 PTS_IIB_glc
431 498 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
335 357 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
170 192 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
323 327 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
231 253 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
437 467 Pfam PF00367 phosphotransferase system, EIIB
437 467 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
219 229 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
150 169 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
405 510 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
382 404 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
55 77 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
273 295 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
126 149 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
33 51 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
230 246 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
382 404 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
84 106 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
351 370 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
308 330 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
431 510 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
431 510 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
196 218 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
52 73 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
276 294 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
295 305 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
432 507 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
432 507 InterPro IPR036878 Glucose permease domain IIB
328 344 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
436 505 SUPERFAMILY SSF55604 Glucose permease domain IIB
436 505 InterPro IPR036878 Glucose permease domain IIB
11 346 Pfam PF02378 Phosphotransferase system, EIIC
11 346 InterPro IPR003352 Phosphotransferase system, EIIC
5 505 PANTHER PTHR30009 CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
405 492 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
405 492 InterPro IPR001996 Phosphotransferase system, IIB component, type 1

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GTE9
AlphaFold — — full sequence — Viewing
ColabFold VK055_0799
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
21 0.736

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 22.6 0.882
2 12.77 0.661
3 5.81 0.283
4 2.78 0.085
5 2.59 0.074