Protein profile

VK055_0888

lacY lactose MFS transporter

Genome: KpATCC43816

Gene: lacY AIK79511.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPL5
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Amino acids 421
Annotations 7
Features 68
PDB binders 0
Druggability 0.697

Overview

Basic information about this protein and its source genome.

Accession
VK055_0888
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
lacY AIK79511.1
Status
annotated
Amino acids
421
Structure source
AlphaFold + ColabFold
GO
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y. GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in). GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
62.205
DEG E-value
6.43e-176
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.09

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.697
Structure A0A0H3GPL5
Pocket Pocket 27
P2Rank 0.967
Structure A0A0H3GPL5
Pocket Pocket 1
ColabFold model
FPocket 0.87 · Pocket 6
P2Rank 0.964 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 57 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.
  • GO:0015528 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: lactose(out) + H+(out) = lactose(in) + H+(in).

Sequence Features

Domain/signature hits from InterPro and related databases.

68 records
Show feature table
Start End DB Term Name
406 421 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
146 167 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
309 331 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
82 103 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
69 83 ProSitePatterns PS00896 LacY family proton/sugar symporters signature 1.
69 83 InterPro IPR018457 LacY/RafB permease family, conserved site
354 374 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
211 418 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
211 418 InterPro IPR036259 MFS transporter superfamily
109 134 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
386 408 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
52 70 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
296 315 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
71 81 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
73 102 PRINTS PR00174 LacY proton/sugar symporter family signature
73 102 InterPro IPR000576 LacY/RafB permease family
265 286 PRINTS PR00174 LacY proton/sugar symporter family signature
265 286 InterPro IPR000576 LacY/RafB permease family
219 247 PRINTS PR00174 LacY proton/sugar symporter family signature
219 247 InterPro IPR000576 LacY/RafB permease family
289 315 PRINTS PR00174 LacY proton/sugar symporter family signature
289 315 InterPro IPR000576 LacY/RafB permease family
150 168 PRINTS PR00174 LacY proton/sugar symporter family signature
150 168 InterPro IPR000576 LacY/RafB permease family
320 339 PRINTS PR00174 LacY proton/sugar symporter family signature
320 339 InterPro IPR000576 LacY/RafB permease family
351 371 PRINTS PR00174 LacY proton/sugar symporter family signature
351 371 InterPro IPR000576 LacY/RafB permease family
108 130 PRINTS PR00174 LacY proton/sugar symporter family signature
108 130 InterPro IPR000576 LacY/RafB permease family
12 405 NCBIfam TIGR00882 oligosaccharide MFS transporter
12 405 InterPro IPR000576 LacY/RafB permease family
135 145 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 409 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
13 409 InterPro IPR020846 Major facilitator superfamily domain
245 263 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
321 342 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
386 405 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
351 373 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
50 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
7 409 Pfam PF01306 LacY proton/sugar symporter
7 409 InterPro IPR000576 LacY/RafB permease family
227 244 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 409 PANTHER PTHR23522 BLL5896 PROTEIN
150 169 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
27 405 CDD cd06172 MFS_LacY
266 288 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
108 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
285 295 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
168 172 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
33 51 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
173 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
192 226 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
104 108 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
9 406 SUPERFAMILY SSF103473 MFS general substrate transporter
9 406 InterPro IPR036259 MFS transporter superfamily
375 385 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
81 103 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
173 192 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
264 284 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
227 244 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 210 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
10 210 InterPro IPR036259 MFS transporter superfamily
343 353 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
316 320 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPL5
AlphaFold — — full sequence — Viewing
ColabFold VK055_0888
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
27 0.697
7 0.336

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 37.23 0.957
2 2.68 0.079
3 2.0 0.043
4 1.95 0.041
5 1.85 0.036