Protein profile

VK055_0924

dethiobiotin synthetase subunit dethiobiotin synthetase

Genome: KpATCC43816

Gene: AIK79548.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GX28
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Amino acids 192
Annotations 7
Features 11
PDB binders 0
Druggability 0.078

Overview

Basic information about this protein and its source genome.

Accession
VK055_0924
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK79548.1
Status
annotated
Amino acids
192
Structure source
AlphaFold + ColabFold
EC
6.3.3.3
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0009102 The chemical reactions and pathways resulting in the formation of biotin, cis-tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid. GO:0000287 Binding to a magnesium (Mg) ion. GO:0004141 Catalysis of the reaction: 7,8-diaminononanoate + ATP + CO2 = ADP + dethiobiotin + 4 H+ + phosphate. GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0042803 Binding to an identical protein to form a homodimer.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
41.361
DEG E-value
1e-30
Localization
Cytoplasmic
ColabFold pLDDT
90.29

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.078
Structure A0A0H3GX28
Pocket Pocket 13
P2Rank 0.418
Structure A0A0H3GX28
Pocket Pocket 1
ColabFold model
FPocket 0.568 · Pocket 1
P2Rank 0.362 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 110 / 4744 genomes with a hit
Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0009102 The chemical reactions and pathways resulting in the formation of biotin, cis-tetrahydro-2-oxothieno(3,4-d)imidazoline-4-valeric acid.
  • GO:0000287 Binding to a magnesium (Mg) ion.
  • GO:0004141 Catalysis of the reaction: 7,8-diaminononanoate + ATP + CO2 = ADP + dethiobiotin + 4 H+ + phosphate.
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0042803 Binding to an identical protein to form a homodimer.

Sequence Features

Domain/signature hits from InterPro and related databases.

11 records
Show feature table
Start End DB Term Name
1 186 PIRSF PIRSF006755 DTB_synth
1 186 InterPro IPR004472 Dethiobiotin synthase BioD
1 181 Gene3D G3DSA:3.40.50.300 -
1 181 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
7 183 PANTHER PTHR43210 DETHIOBIOTIN SYNTHETASE
7 183 InterPro IPR004472 Dethiobiotin synthase BioD
9 151 CDD cd03109 DTBS
1 181 FunFam G3DSA:3.40.50.300:FF:000292 ATP-dependent dethiobiotin synthetase BioD
4 173 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
4 173 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
12 168 Pfam PF13500 AAA domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GX28
AlphaFold — — full sequence — Viewing
ColabFold VK055_0924
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 7.12 0.369
2 1.8 0.034