Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: VK055_0995
Assembly: Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene: AIK79618.1
Status: annotated
Amino acids: 379
Structure source: ColabFold

GO

GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Human identity (%): 0.0
Gut microbiome off-target: hit
Essential (DEG): Y
DEG identity (%): 45.833
DEG E-value: 3.73e-08
Localization: CytoplasmicMembrane
ColabFold pLDDT: 89.22

Selected Druggability evidence

ColabFold / curated model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.924

Structure CB_VK055_0995

Pocket Pocket 3

P2Rank 0.236

Structure CB_VK055_0995

Pocket Pocket 1

ColabFold model

FPocket 0.924 · Pocket 3

P2Rank 0.236 · Pocket 1

Core conservation Accessory gene

Roary core

CoreCruncher accessory

Gut microbiome 111 / 4744 genomes with a hit

Normalized 0.023

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3

GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

36 records

Show feature table

Start	End	DB	Term	Name
269	360	NCBIfam	TIGR00826	glucose PTS transporter subunit EIIB
269	360	InterPro	IPR001996	Phosphotransferase system, IIB component, type 1
202	223	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
32	54	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
303	335	Pfam	PF00367	phosphotransferase system, EIIB
303	335	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
31	54	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
230	248	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
297	374	FunFam	G3DSA:3.30.1360.60:FF:000001	PTS system glucose-specific IIBC component PtsG
298	379	ProSiteProfiles	PS51098	PTS_EIIB type-1 domain profile.
298	379	InterPro	IPR001996	Phosphotransferase system, IIB component, type 1
313	330	ProSitePatterns	PS01035	PTS EIIB domains cysteine phosphorylation site signature.
313	330	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
197	201	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
274	379	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
224	229	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
172	194	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
303	375	SUPERFAMILY	SSF55604	Glucose permease domain IIB
303	375	InterPro	IPR036878	Glucose permease domain IIB
173	196	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
201	223	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
301	374	CDD	cd00212	PTS_IIB_glc
301	374	InterPro	IPR018113	Phosphotransferase system EIIB, cysteine phosphorylation site
299	376	Gene3D	G3DSA:3.30.1360.60	Glucose permease domain IIB
299	376	InterPro	IPR036878	Glucose permease domain IIB
145	162	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	210	Pfam	PF02378	Phosphotransferase system, EIIC
1	210	InterPro	IPR003352	Phosphotransferase system, EIIC
254	273	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	30	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1	376	PANTHER	PTHR30009	CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
249	253	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
55	172	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
246	268	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	280	ProSiteProfiles	PS51103	PTS_EIIC type-1 domain profile.
1	280	InterPro	IPR013013	Phosphotransferase system, EIIC component, type 1

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
ColabFold VK055_0995	ColabFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
3				0.924
12				0.568

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK	Sticks	Spheres	Surfaces	Score	Probability	Labels	Zoom	Positions
1				3.28	0.114
2				1.11	0.008