Protein profile

VK055_1255

signal peptide peptidase SppA, 67K type

Genome: KpATCC43816

Gene: AIK79878.1 sppA Structure source: AlphaFold + ColabFold UniProt A0A0H3GVV4
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Amino acids 617
Annotations 6
Features 29
PDB binders 0
Druggability 0.492

Overview

Basic information about this protein and its source genome.

Accession
VK055_1255
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK79878.1 sppA
Status
annotated
Amino acids
617
Structure source
AlphaFold + ColabFold
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid. GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds. GO:0006465 OBSOLETE. The proteolytic removal of a signal peptide from a protein during or after transport to a specific location in the cell. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0008236 Catalysis of the hydrolysis of peptide bonds in a polypeptide chain by a catalytic mechanism that involves a catalytic triad consisting of a serine nucleophile that is activated by a proton relay involving an acidic residue (e.g. aspartate or glutamate) and a basic residue (usually histidine).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
41.955
DEG E-value
3.39e-95
Localization
CytoplasmicMembrane
ColabFold pLDDT
89.34

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.492
Structure A0A0H3GVV4
Pocket Pocket 45
P2Rank 0.459
Structure A0A0H3GVV4
Pocket Pocket 1
ColabFold model
FPocket 0.925 · Pocket 36
P2Rank 0.426 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 105 / 4744 genomes with a hit
Normalized 0.022

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
  • GO:0006465 OBSOLETE. The proteolytic removal of a signal peptide from a protein during or after transport to a specific location in the cell.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0008236 Catalysis of the hydrolysis of peptide bonds in a polypeptide chain by a catalytic mechanism that involves a catalytic triad consisting of a serine nucleophile that is activated by a proton relay involving an acidic residue (e.g. aspartate or glutamate) and a basic residue (usually histidine).

Sequence Features

Domain/signature hits from InterPro and related databases.

29 records
Show feature table
Start End DB Term Name
8 617 PIRSF PIRSF001217 Protease_4_SppA
8 617 InterPro IPR004634 Peptidase S49, protease IV
21 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
318 550 Gene3D G3DSA:3.90.226.10 -
326 536 CDD cd07019 S49_SppA_1
326 536 InterPro IPR033854 Signal peptide peptidase A
33 286 SUPERFAMILY SSF52096 ClpP/crotonase
33 286 InterPro IPR029045 ClpP/crotonase-like domain superfamily
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
55 217 FunFam G3DSA:3.90.226.10:FF:000051 Protease 4
58 296 CDD cd07018 S49_SppA_67K_type
58 296 InterPro IPR047217 Signal peptide peptidase A, N-terminal
43 617 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
53 593 PANTHER PTHR33209 PROTEASE 4
327 540 NCBIfam TIGR00706 signal peptide peptidase SppA
327 540 InterPro IPR004635 Peptidase S49, SppA
16 608 NCBIfam TIGR00705 signal peptide peptidase SppA, 67K type
16 608 InterPro IPR004634 Peptidase S49, protease IV
320 452 FunFam G3DSA:3.90.226.10:FF:000033 Protease 4
140 296 Pfam PF01343 Peptidase family S49
140 296 InterPro IPR002142 Peptidase S49
391 542 Pfam PF01343 Peptidase family S49
391 542 InterPro IPR002142 Peptidase S49
432 496 Gene3D G3DSA:6.20.330.10 -
323 529 SUPERFAMILY SSF52096 ClpP/crotonase
323 529 InterPro IPR029045 ClpP/crotonase-like domain superfamily
55 217 Gene3D G3DSA:3.90.226.10 -
218 317 Gene3D G3DSA:3.90.226.10 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GVV4
AlphaFold — — full sequence — Viewing
ColabFold VK055_1255
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
45 0.492
35 0.146
37 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 9.94 0.459
2 9.27 0.425
3 8.56 0.389
4 5.08 0.191
5 5.03 0.188