Protein profile

VK055_2129

high-affinity nickel-transport family protein

Genome: KpATCC43816

Gene: AIK80734.1 Structure source: AlphaFold + ColabFold UniProt A0A483G0U1
Export view
Amino acids 287
Annotations 7
Features 25
PDB binders 0
Druggability 0.99

Overview

Basic information about this protein and its source genome.

Accession
VK055_2129
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK80734.1
Status
annotated
Amino acids
287
Structure source
AlphaFold + ColabFold
GO
GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore. GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0046583 Enables the transfer of a cation or cations from the inside of the cell to the outside of the cell across a membrane. GO:0006824 The directed movement of cobalt (Co2+) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0032025 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cobalt ion (Co2+) stimulus.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
23.022
Localization
CytoplasmicMembrane
ColabFold pLDDT
72.35

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.99
Structure A0A483G0U1
Pocket Pocket 15
P2Rank 0.883
Structure A0A483G0U1
Pocket Pocket 1
ColabFold model
FPocket 0.768 · Pocket 2
P2Rank 0.514 · Pocket 1
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 45 / 4744 genomes with a hit
Normalized 0.009

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0035444 The directed movement of nickel (Ni) cations across a membrane by means of some agent such as a transporter or pore.
  • GO:0015099 Enables the transfer of nickel (Ni) cations from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0046583 Enables the transfer of a cation or cations from the inside of the cell to the outside of the cell across a membrane.
  • GO:0006824 The directed movement of cobalt (Co2+) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0032025 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cobalt ion (Co2+) stimulus.
  • GO:0010045 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nickel cation stimulus.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
89 107 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
266 284 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
285 287 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
52 77 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
190 211 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 123 SUPERFAMILY SSF103473 MFS general substrate transporter
10 123 InterPro IPR036259 MFS transporter superfamily
212 222 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
7 283 PANTHER PTHR40659 NICKEL/COBALT EFFLUX SYSTEM RCNA
53 75 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
264 286 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
90 107 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
118 152 MobiDBLite mobidb-lite consensus disorder prediction
246 265 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
10 32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
78 88 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
13 284 Pfam PF03824 High-affinity nickel-transport protein
13 284 InterPro IPR011541 Nickel/cobalt transporter, high-affinity
223 245 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
32 51 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
190 212 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
222 244 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
108 189 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A483G0U1
AlphaFold — — full sequence — Viewing
ColabFold VK055_2129
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
15 0.99
2 0.741
27 0.478
23 0.214

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 11.61 0.618
2 6.17 0.307
3 5.86 0.287
4 3.54 0.131
5 1.52 0.022