Protein profile

VK055_2164

protoheme IX farnesyltransferase

Genome: KpATCC43816

Gene: AIK80769.1 cyoE Structure source: AlphaFold + ColabFold UniProt A0A0H3GKG4
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Amino acids 295
Annotations 7
Features 42
PDB binders 0
Druggability 0.857

Overview

Basic information about this protein and its source genome.

Accession
VK055_2164
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK80769.1 cyoE
Status
annotated
Amino acids
295
Structure source
AlphaFold + ColabFold
EC
2.5.1.141
GO
GO:0016765 Catalysis of the transfer of an alkyl or aryl (but not methyl) group from one compound (donor) to another (acceptor). GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008495 Catalysis of the reaction: protoheme IX + (2E,6E)-farnesyl diphosphate + H2O = heme O + diphosphate. GO:0006783 The chemical reactions and pathways resulting in the formation of heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring, from less complex precursors. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0048034 The chemical reactions and pathways resulting in the formation of heme O, a derivative of heme containing a 17-carbon hydroxyethylfarnesyl side chain at position 8 of the tetrapyrrole macrocycle.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
37.5
Human E-value
3.73e-27
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
42.857
Localization
CytoplasmicMembrane
ColabFold pLDDT
95.27

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.857
Structure A0A0H3GKG4
Pocket Pocket 1
P2Rank 0.984
Structure A0A0H3GKG4
Pocket Pocket 1
ColabFold model
FPocket 0.927 · Pocket 2
P2Rank 0.973 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 125 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 6 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

6
  • GO:0016765 Catalysis of the transfer of an alkyl or aryl (but not methyl) group from one compound (donor) to another (acceptor).
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008495 Catalysis of the reaction: protoheme IX + (2E,6E)-farnesyl diphosphate + H2O = heme O + diphosphate.
  • GO:0006783 The chemical reactions and pathways resulting in the formation of heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring, from less complex precursors.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0048034 The chemical reactions and pathways resulting in the formation of heme O, a derivative of heme containing a 17-carbon hydroxyethylfarnesyl side chain at position 8 of the tetrapyrrole macrocycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

42 records
Show feature table
Start End DB Term Name
263 284 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
3 281 NCBIfam TIGR01473 heme o synthase
3 281 InterPro IPR006369 Protohaem IX farnesyltransferase
160 182 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
18 267 Pfam PF01040 UbiA prenyltransferase family
18 267 InterPro IPR000537 UbiA prenyltransferase family
156 160 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
13 181 Gene3D G3DSA:1.10.357.140 UbiA prenyltransferase
13 181 InterPro IPR044878 UbiA prenyltransferase superfamily
107 126 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
80 102 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
133 155 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
127 132 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
232 251 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
208 230 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
37 59 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
37 59 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
56 78 ProSitePatterns PS00943 UbiA prenyltransferase family signature.
56 78 InterPro IPR030470 UbiA prenyltransferase conserved site
263 285 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
285 295 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
161 182 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
208 226 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
234 251 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 281 PANTHER PTHR43448 PROTOHEME IX FARNESYLTRANSFERASE, MITOCHONDRIAL
107 126 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
227 231 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
32 36 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
9 163 FunFam G3DSA:1.10.357.140:FF:000001 Protoheme IX farnesyltransferase
133 155 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 283 Hamap MF_00154 Protoheme IX farnesyltransferase [cyoE].
1 283 InterPro IPR006369 Protohaem IX farnesyltransferase
80 101 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
6 277 CDD cd13957 PT_UbiA_Cox10
6 277 InterPro IPR006369 Protohaem IX farnesyltransferase
102 106 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
252 262 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
60 79 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
183 207 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GKG4
AlphaFold — — full sequence — Viewing
ColabFold VK055_2164
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.857

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 40.73 0.964
2 3.83 0.149
3 3.36 0.119
4 1.78 0.033
5 1.59 0.025