Protein profile

VK055_2814

PTS system, cellobiose-specific IIC component

Genome: KpATCC43816

Gene: AIK81403.1 celB2 Structure source: AlphaFold + ColabFold UniProt A0A0H3GHH9
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Amino acids 439
Annotations 5
Features 46
PDB binders 0
Druggability 0.974

Overview

Basic information about this protein and its source genome.

Accession
VK055_2814
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81403.1 celB2
Status
annotated
Amino acids
439
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:1901264 The directed movement of a carbohydrate derivative into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
29.825
Localization
CytoplasmicMembrane
ColabFold pLDDT
92.96

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.974
Structure A0A0H3GHH9
Pocket Pocket 10
P2Rank 0.709
Structure A0A0H3GHH9
Pocket Pocket 1
ColabFold model
FPocket 0.896 · Pocket 2
P2Rank 0.676 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 22 / 4744 genomes with a hit
Normalized 0.005

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:1901264 The directed movement of a carbohydrate derivative into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

46 records
Show feature table
Start End DB Term Name
333 355 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
196 200 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
132 154 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 302 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
201 219 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
61 81 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
389 409 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 422 NCBIfam TIGR00410 PTS system, lactose/cellobiose family IIC component
1 422 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
61 83 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
42 60 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
362 383 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
279 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
82 92 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
175 195 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 432 PIRSF PIRSF006351 PTS_EIIC-Cellobiose
1 432 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
1 409 ProSiteProfiles PS51105 PTS_EIIC type-3 domain profile.
1 409 InterPro IPR004501 Phosphotransferase system, EIIC component, type 3
93 112 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
95 117 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
384 388 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
362 384 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
226 250 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
218 240 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 422 NCBIfam TIGR00359 PTS cellobiose transporter subunit IIC
1 422 InterPro IPR004796 Phosphotransferase system, cellobiose-type IIC component
155 174 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
132 154 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
314 332 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
333 337 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 19 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
2 427 PANTHER PTHR33989 -
113 131 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
20 41 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
303 313 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
174 196 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
356 361 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
388 410 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
251 281 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
338 355 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
19 41 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
17 347 Pfam PF02378 Phosphotransferase system, EIIC
17 347 InterPro IPR003352 Phosphotransferase system, EIIC
410 439 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
220 225 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GHH9
AlphaFold — — full sequence — Viewing
ColabFold VK055_2814
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
10 0.974
2 0.901
5 0.236

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.6 0.569
2 4.82 0.214
3 3.26 0.113
4 1.36 0.016
5 0.98 0.005