Protein profile

VK055_3273

lacY lactose MFS transporter

Genome: KpATCC43816

Gene: AIK81834.1 lacY4 Structure source: AlphaFold + ColabFold UniProt A0A0H3GPM5
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Amino acids 421
Annotations 7
Features 71
PDB binders 0
Druggability 0.709

Overview

Basic information about this protein and its source genome.

Accession
VK055_3273
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81834.1 lacY4
Status
annotated
Amino acids
421
Structure source
AlphaFold + ColabFold
GO
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y. GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in). GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
58.481
DEG E-value
1.05e-169
Localization
CytoplasmicMembrane
ColabFold pLDDT
84.98

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.709
Structure A0A0H3GPM5
Pocket Pocket 1
P2Rank 0.956
Structure A0A0H3GPM5
Pocket Pocket 1
ColabFold model
FPocket 0.836 · Pocket 24
P2Rank 0.952 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 57 / 4744 genomes with a hit
Normalized 0.012

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.
  • GO:0015528 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: lactose(out) + H+(out) = lactose(in) + H+(in).

Sequence Features

Domain/signature hits from InterPro and related databases.

71 records
Show feature table
Start End DB Term Name
9 210 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
9 210 InterPro IPR036259 MFS transporter superfamily
386 406 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
69 83 ProSitePatterns PS00896 LacY family proton/sugar symporters signature 1.
69 83 InterPro IPR018457 LacY/RafB permease family, conserved site
354 374 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
265 286 PRINTS PR00174 LacY proton/sugar symporter family signature
265 286 InterPro IPR000576 LacY/RafB permease family
320 339 PRINTS PR00174 LacY proton/sugar symporter family signature
320 339 InterPro IPR000576 LacY/RafB permease family
289 315 PRINTS PR00174 LacY proton/sugar symporter family signature
289 315 InterPro IPR000576 LacY/RafB permease family
219 247 PRINTS PR00174 LacY proton/sugar symporter family signature
219 247 InterPro IPR000576 LacY/RafB permease family
108 130 PRINTS PR00174 LacY proton/sugar symporter family signature
108 130 InterPro IPR000576 LacY/RafB permease family
150 168 PRINTS PR00174 LacY proton/sugar symporter family signature
150 168 InterPro IPR000576 LacY/RafB permease family
351 371 PRINTS PR00174 LacY proton/sugar symporter family signature
351 371 InterPro IPR000576 LacY/RafB permease family
73 102 PRINTS PR00174 LacY proton/sugar symporter family signature
73 102 InterPro IPR000576 LacY/RafB permease family
296 315 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
229 251 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
130 149 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
51 71 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
407 421 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
327 346 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
245 263 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
173 192 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
83 101 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
321 342 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
295 317 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 415 PANTHER PTHR23522 BLL5896 PROTEIN
150 167 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
50 72 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
227 244 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
9 410 Pfam PF01306 LacY proton/sugar symporter
9 410 InterPro IPR000576 LacY/RafB permease family
193 226 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
9 410 SUPERFAMILY SSF103473 MFS general substrate transporter
9 410 InterPro IPR036259 MFS transporter superfamily
11 405 NCBIfam TIGR00882 oligosaccharide MFS transporter
11 405 InterPro IPR000576 LacY/RafB permease family
150 169 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
285 299 ProSitePatterns PS00897 LacY family proton/sugar symporters signature 2.
285 299 InterPro IPR018457 LacY/RafB permease family, conserved site
107 129 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
266 288 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
108 130 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
72 82 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
285 295 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
353 375 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
168 172 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
40 50 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
14 405 CDD cd06172 MFS_LacY
375 385 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
13 409 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
13 409 InterPro IPR020846 Major facilitator superfamily domain
81 103 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
173 192 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
102 106 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
264 284 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 39 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
343 353 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
385 407 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
211 421 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
211 421 InterPro IPR036259 MFS transporter superfamily
316 320 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GPM5
AlphaFold — — full sequence — Viewing
ColabFold VK055_3273
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.709
5 0.701

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 31.86 0.941
2 1.46 0.02
3 1.21 0.011
4 0.75 0.002