Protein profile

VK055_3379

PTS system, alpha-glucoside-specific IIBC component domain protein

Genome: KpATCC43816

Gene: AIK81936.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GZ00
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Amino acids 540
Annotations 6
Features 58
PDB binders 0
Druggability 0.742

Overview

Basic information about this protein and its source genome.

Accession
VK055_3379
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK81936.1
Status
annotated
Amino acids
540
Structure source
AlphaFold + ColabFold
GO
GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
37.885
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.65

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.742
Structure A0A0H3GZ00
Pocket Pocket 8
P2Rank 0.977
Structure A0A0H3GZ00
Pocket Pocket 1
ColabFold model
FPocket 0.746 · Pocket 7
P2Rank 0.936 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 155 / 4744 genomes with a hit
Normalized 0.033

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008982 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein N-phosphohistidine + sugar(out) = protein histidine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.

Sequence Features

Domain/signature hits from InterPro and related databases.

58 records
Show feature table
Start End DB Term Name
352 371 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
405 540 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
164 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
128 150 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
203 222 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 420 ProSiteProfiles PS51103 PTS_EIIC type-1 domain profile.
1 420 InterPro IPR013013 Phosphotransferase system, EIIC component, type 1
129 152 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
383 404 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
153 163 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
234 255 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
305 323 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
344 366 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
329 345 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
299 304 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
162 184 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
10 32 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
199 221 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
454 483 Pfam PF00367 phosphotransferase system, EIIB
454 483 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
256 274 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
275 298 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
10 346 Pfam PF02378 Phosphotransferase system, EIIC
10 346 InterPro IPR003352 Phosphotransferase system, EIIC
1 526 PANTHER PTHR30009 CYTOCHROME C-TYPE SYNTHESIS PROTEIN AND PTS TRANSMEMBRANE COMPONENT
110 128 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
346 351 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
233 255 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 524 NCBIfam TIGR02005 alpha-glucoside-specific PTS transporter subunit IIBC
2 524 InterPro IPR010975 Phosphotransferase system, alpha-glucoside-specific IIBC component
448 530 ProSiteProfiles PS51098 PTS_EIIB type-1 domain profile.
448 530 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
192 202 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
387 409 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
449 527 Gene3D G3DSA:3.30.1360.60 Glucose permease domain IIB
449 527 InterPro IPR036878 Glucose permease domain IIB
60 358 NCBIfam TIGR00852 maltose/glucose-specific PTS transporter subunit IIC
60 358 InterPro IPR004719 Phosphotransferase system, maltose/glucose-specific subfamily IIC component
307 329 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
409 510 NCBIfam TIGR00826 glucose PTS transporter subunit EIIB
409 510 InterPro IPR001996 Phosphotransferase system, IIB component, type 1
372 382 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
450 524 CDD cd00212 PTS_IIB_glc
450 524 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
223 233 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
33 62 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
81 86 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
278 300 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
91 113 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
87 109 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
324 328 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
463 480 ProSitePatterns PS01035 PTS EIIB domains cysteine phosphorylation site signature.
463 480 InterPro IPR018113 Phosphotransferase system EIIB, cysteine phosphorylation site
63 80 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
451 524 SUPERFAMILY SSF55604 Glucose permease domain IIB
451 524 InterPro IPR036878 Glucose permease domain IIB

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GZ00
AlphaFold — — full sequence — Viewing
ColabFold VK055_3379
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
8 0.742
1 0.591
12 0.202

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 40.07 0.963
2 7.72 0.409
3 4.42 0.186
4 2.27 0.057
5 2.18 0.052