Protein profile

VK055_4201

emrA Membrane Fusion Protein

Genome: KpATCC43816

Gene: AIK82746.1 Structure source: AlphaFold + ColabFold UniProt A0A377W0F8
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Amino acids 388
Annotations 6
Features 15
PDB binders 0
Druggability 0.584

Overview

Basic information about this protein and its source genome.

Accession
VK055_4201
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK82746.1
Status
annotated
Amino acids
388
Structure source
AlphaFold + ColabFold
GO
GO:0042910 Enables the directed movement of a xenobiotic from one side of a membrane to the other. A xenobiotic is a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical. GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
54.211
DEG E-value
5.489999999999999e-153
Localization
CytoplasmicMembrane
ColabFold pLDDT
90.34

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.584
Structure A0A377W0F8
Pocket Pocket 3
P2Rank
Structure A0A377W0F8
Pocket No pockets
ColabFold model
FPocket 0.538 · Pocket 11
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 105 / 4744 genomes with a hit
Normalized 0.022

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0042910 Enables the directed movement of a xenobiotic from one side of a membrane to the other. A xenobiotic is a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
  • GO:1990961 A process that reduces or removes the toxicity of a xenobiotic by exporting it outside the cell.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015721 The directed movement of bile acid and bile salts into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.

Sequence Features

Domain/signature hits from InterPro and related databases.

15 records
Show feature table
Start End DB Term Name
43 388 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
33 343 Pfam PF00529 Cation efflux system protein CusB domain 1
33 343 InterPro IPR043602 Cation efflux system protein CusB, domain 1
21 42 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
15 352 PANTHER PTHR30386 MEMBRANE FUSION SUBUNIT OF EMRAB-TOLC MULTIDRUG EFFLUX PUMP
124 144 Coils Coil Coil
191 265 SUPERFAMILY SSF111369 HlyD-like secretion proteins
1 20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
18 342 NCBIfam TIGR00998 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit
18 342 InterPro IPR005694 Membrane fusion proteins, proteobacteria
230 352 Gene3D G3DSA:2.40.30.170 -
230 352 FunFam G3DSA:2.40.30.170:FF:000003 Multidrug resistance protein A
210 290 Pfam PF16576 Barrel-sandwich domain of CusB or HlyD membrane-fusion
210 290 InterPro IPR032317 RND efflux pump, membrane fusion protein, barrel-sandwich domain
21 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A377W0F8
AlphaFold — — full sequence — Viewing
ColabFold VK055_4201
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.584
17 0.373
1 0.012
4 0.002