Protein profile

VK055_4262

lacY lactose MFS transporter

Genome: KpATCC43816

Gene: AIK82807.1 lacY5 Structure source: AlphaFold + ColabFold UniProt A0A0H3GSQ6
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Amino acids 413
Annotations 7
Features 69
PDB binders 0
Druggability 0.436

Overview

Basic information about this protein and its source genome.

Accession
VK055_4262
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK82807.1 lacY5
Status
annotated
Amino acids
413
Structure source
AlphaFold + ColabFold
GO
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y. GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in). GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
42.818
DEG E-value
5.879999999999999e-94
Localization
CytoplasmicMembrane
ColabFold pLDDT
82.77

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.436
Structure A0A0H3GSQ6
Pocket Pocket 29
P2Rank 0.952
Structure A0A0H3GSQ6
Pocket Pocket 1
ColabFold model
FPocket 0.66 · Pocket 4
P2Rank 0.955 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 65 / 4744 genomes with a hit
Normalized 0.014

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008643 The directed movement of carbohydrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Carbohydrates are a group of organic compounds based of the general formula Cx(H2O)y.
  • GO:0005351 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: carbohydrate(out) + H+(out) = carbohydrate(in) + H+(in).
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0030395 Binding to lactose, a disaccharide of glucose and galactose, the carbohydrate of milk.
  • GO:0015528 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: lactose(out) + H+(out) = lactose(in) + H+(in).

Sequence Features

Domain/signature hits from InterPro and related databases.

69 records
Show feature table
Start End DB Term Name
257 279 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
311 332 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
280 306 PRINTS PR00174 LacY proton/sugar symporter family signature
280 306 InterPro IPR000576 LacY/RafB permease family
311 330 PRINTS PR00174 LacY proton/sugar symporter family signature
311 330 InterPro IPR000576 LacY/RafB permease family
103 125 PRINTS PR00174 LacY proton/sugar symporter family signature
103 125 InterPro IPR000576 LacY/RafB permease family
69 98 PRINTS PR00174 LacY proton/sugar symporter family signature
69 98 InterPro IPR000576 LacY/RafB permease family
256 277 PRINTS PR00174 LacY proton/sugar symporter family signature
256 277 InterPro IPR000576 LacY/RafB permease family
145 163 PRINTS PR00174 LacY proton/sugar symporter family signature
145 163 InterPro IPR000576 LacY/RafB permease family
211 239 PRINTS PR00174 LacY proton/sugar symporter family signature
211 239 InterPro IPR000576 LacY/RafB permease family
342 362 PRINTS PR00174 LacY proton/sugar symporter family signature
342 362 InterPro IPR000576 LacY/RafB permease family
377 396 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
366 376 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
213 235 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
210 235 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
1 400 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
1 400 InterPro IPR020846 Major facilitator superfamily domain
1 32 Phobius SIGNAL_PEPTIDE Signal peptide region
45 67 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
101 125 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
190 209 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
145 164 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 400 PANTHER PTHR23522 BLL5896 PROTEIN
286 305 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
170 189 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
103 125 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 197 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
6 197 InterPro IPR036259 MFS transporter superfamily
280 285 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
165 169 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
397 413 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
77 95 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
345 367 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
8 400 SUPERFAMILY SSF103473 MFS general substrate transporter
8 400 InterPro IPR036259 MFS transporter superfamily
96 100 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
25 32 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
76 98 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
126 145 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
203 407 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
203 407 InterPro IPR036259 MFS transporter superfamily
68 76 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
146 164 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
236 258 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
333 343 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
168 187 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
306 310 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
33 47 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
48 67 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
286 305 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
9 396 NCBIfam TIGR00882 oligosaccharide MFS transporter
9 396 InterPro IPR000576 LacY/RafB permease family
377 396 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
310 332 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 400 Pfam PF01306 LacY proton/sugar symporter
9 400 InterPro IPR000576 LacY/RafB permease family
344 365 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
12 24 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
259 279 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 27 SignalP_EUK SignalP-TM SignalP-TM

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GSQ6
AlphaFold — — full sequence — Viewing
ColabFold VK055_4262
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
36 0.335
35 0.191
27 0.061
16 0.005

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 42.35 0.952
2 5.39 0.21
3 4.85 0.178
4 4.32 0.148
5 3.06 0.081