Protein profile

VK055_4347

membrane-bound lytic murein transglycosylase A

Genome: KpATCC43816

Gene: mltA AIK82890.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GUL3
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Amino acids 352
Annotations 8
Features 20
PDB binders 0
Druggability 0.618

Overview

Basic information about this protein and its source genome.

Accession
VK055_4347
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
mltA AIK82890.1
Status
annotated
Amino acids
352
Structure source
AlphaFold + ColabFold
EC
4.2.2.n1
GO
GO:0019867 The external membrane of Gram-negative bacteria or certain organelles such as mitochondria and chloroplasts; freely permeable to most ions and metabolites. GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond. GO:0009254 The continual breakdown and regeneration of peptidoglycan required to maintain the bacterial cell wall. Peptidoglycans consist of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides. GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures. GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
Y
DEG identity (%)
94.318
DEG E-value
0.0
Localization
OuterMembrane
ColabFold pLDDT
93.12

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.618
Structure A0A0H3GUL3
Pocket Pocket 1
P2Rank 0.409
Structure A0A0H3GUL3
Pocket Pocket 1
ColabFold model
FPocket 0.309 · Pocket 16
P2Rank 0.816 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 124 / 4744 genomes with a hit
Normalized 0.026

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0019867 The external membrane of Gram-negative bacteria or certain organelles such as mitochondria and chloroplasts; freely permeable to most ions and metabolites.
  • GO:0004553 Catalysis of the hydrolysis of any O-glycosyl bond.
  • GO:0009254 The continual breakdown and regeneration of peptidoglycan required to maintain the bacterial cell wall. Peptidoglycans consist of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0009279 A lipid bilayer that forms the outermost membrane of the cell envelope; enriched in polysaccharide and protein; the outer leaflet of the membrane contains specific lipopolysaccharide structures.
  • GO:0008933 Catalysis of the cleavage of a peptidoglycan chain into a peptidoglycan chain with N-acetyl-1,6-anhydromuramyl-[peptide] at the reducing end + a peptidoglycan chain with N-acetylglucosamine at the non-reducing end. Includes endolytic transglycosylase activity that fragments the glycan chain internally and exolytic transgylcosylase activity that cleaves a terminal disaccharide from the end of the glycan strand.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
30 341 Gene3D G3DSA:2.40.40.10 -
30 341 InterPro IPR036908 RlpA-like domain superfamily
112 244 SMART SM00925 MltA_2
112 244 InterPro IPR005300 Lytic transglycosylase MltA, domain B
2 349 PANTHER PTHR30124 MEMBRANE-BOUND LYTIC MUREIN TRANSGLYCOSYLASE A
2 349 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A
12 340 SUPERFAMILY SSF50685 Barwin-like endoglucanases
12 340 InterPro IPR036908 RlpA-like domain superfamily
112 249 FunFam G3DSA:2.40.240.50:FF:000001 Membrane-bound lytic murein transglycosylase A
113 246 CDD cd14472 mltA_B_like
248 340 CDD cd22785 DPBB_MltA-like
1 134 PIRSF PIRSF019422 MltA
1 134 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A
132 352 PIRSF PIRSF019422 MltA
132 352 InterPro IPR026044 Membrane-bound lytic murein transglycosylase A
112 249 Gene3D G3DSA:2.40.240.50 -
264 341 Pfam PF06725 3D domain
264 341 InterPro IPR010611 3D domain
72 244 Pfam PF03562 MltA specific insert domain
72 244 InterPro IPR005300 Lytic transglycosylase MltA, domain B

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GUL3
AlphaFold — — full sequence — Viewing
ColabFold VK055_4347
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
9 0.042
7 0.001
5 0.0
16 0.0

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 8.91 0.409
2 7.27 0.318
3 2.42 0.053
4 1.92 0.033
5 1.73 0.026