Protein profile

VK055_4776

histidine kinase-, DNA gyrase B-, and HSP90-likeATPase family protein

Genome: KpATCC43816

Gene: AIK83304.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GWF4
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Amino acids 565
Annotations 8
Features 37
PDB binders 0
Druggability 0.881

Overview

Basic information about this protein and its source genome.

Accession
VK055_4776
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK83304.1
Status
annotated
Amino acids
565
Structure source
AlphaFold + ColabFold
EC
2.7.13.3
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0004673 Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine. GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
33.105
Localization
CytoplasmicMembrane
ColabFold pLDDT
86.48

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.881
Structure A0A0H3GWF4
Pocket Pocket 13
P2Rank 0.314
Structure A0A0H3GWF4
Pocket Pocket 1
ColabFold model
FPocket 0.762 · Pocket 1
P2Rank 0.356 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 104 / 4744 genomes with a hit
Normalized 0.022

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0004673 Catalysis of the reaction: ATP + protein L-histidine = ADP + protein phospho-L-histidine.
  • GO:0071555 A process that results in the assembly, arrangement of constituent parts, or disassembly of the cell wall, the rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

Sequence Features

Domain/signature hits from InterPro and related databases.

37 records
Show feature table
Start End DB Term Name
415 559 Gene3D G3DSA:3.30.565.10 -
415 559 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
74 555 PANTHER PTHR34220 SENSOR HISTIDINE KINASE YPDA
227 345 SUPERFAMILY SSF55781 GAF domain-like
141 163 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
173 195 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
77 99 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
140 161 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
414 551 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
414 551 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
76 97 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
27 45 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
109 128 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
7 26 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
98 108 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
46 65 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
162 172 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
452 554 SMART SM00387 HKATPase_4
452 554 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
173 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
45 200 Gene3D G3DSA:1.10.1760.20 -
9 31 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
192 565 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
46 64 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
226 357 Gene3D G3DSA:3.30.450.40 -
226 357 InterPro IPR029016 GAF-like domain superfamily
109 128 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
65 75 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
452 551 CDD cd16955 HATPase_YpdA-like
359 438 Pfam PF06580 Histidine kinase
359 438 InterPro IPR010559 Signal transduction histidine kinase, internal region
129 139 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
456 552 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
456 552 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
29 193 Pfam PF07694 5TMR of 5TMR-LYT
29 193 InterPro IPR011620 Signal transduction histidine kinase, 5TM receptor LytS, transmembrane region

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GWF4
AlphaFold — — full sequence — Viewing
ColabFold VK055_4776
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
13 0.881
20 0.341
31 0.308

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.39 0.184
2 1.92 0.039
3 1.75 0.032
4 1.66 0.028
5 0.55 0.0