Protein profile

VK055_4884

putative 2-component sensor protein

Genome: KpATCC43816

Gene: AIK83411.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GW72
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Amino acids 885
Annotations 6
Features 34
PDB binders 0
Druggability 0.906

Overview

Basic information about this protein and its source genome.

Accession
VK055_4884
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK83411.1
Status
annotated
Amino acids
885
Structure source
AlphaFold + ColabFold
EC
2.7.2.-
GO
GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0016774 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to a carboxyl group (acceptor).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
22.556
Localization
CytoplasmicMembrane
ColabFold pLDDT
83.36

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.906
Structure A0A0H3GW72
Pocket Pocket 1
P2Rank 0.536
Structure A0A0H3GW72
Pocket Pocket 1
ColabFold model
FPocket 0.868 · Pocket 2
P2Rank 0.713 · Pocket 1
Core conservation Accessory gene
Roary core
CoreCruncher accessory
Gut microbiome 71 / 4744 genomes with a hit
Normalized 0.015

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016774 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to a carboxyl group (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

34 records
Show feature table
Start End DB Term Name
303 324 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 15 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
464 674 ProSiteProfiles PS50109 Histidine kinase domain profile.
464 674 InterPro IPR005467 Histidine kinase domain
806 866 Pfam PF01627 Hpt domain
806 866 InterPro IPR008207 Signal transduction histidine kinase, phosphotransfer (Hpt) domain
27 38 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
562 672 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
562 672 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
683 784 Pfam PF16359 RcsD-ABL domain
683 784 InterPro IPR032306 Phosphotransferase RcsD, RcsD-ABL domain
39 302 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
803 884 CDD cd00088 HPT
803 884 InterPro IPR008207 Signal transduction histidine kinase, phosphotransfer (Hpt) domain
797 884 Gene3D G3DSA:1.20.120.160 HPT domain
797 884 InterPro IPR036641 HPT domain superfamily
508 672 Gene3D G3DSA:3.30.565.10 -
508 672 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
504 674 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
504 674 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
682 796 Gene3D G3DSA:3.40.50.11620 -
682 796 InterPro IPR038616 Phosphotransferase RcsD, RcsD-ABL domain superfamily
9 885 Hamap MF_00980 Phosphotransferase RcsD [rcsD].
9 885 InterPro IPR030861 Phosphotransferase RcsD
801 884 SUPERFAMILY SSF47226 Histidine-containing phosphotransfer domain, HPT domain
801 884 InterPro IPR036641 HPT domain superfamily
494 679 PANTHER PTHR43047 TWO-COMPONENT HISTIDINE PROTEIN KINASE
798 885 ProSiteProfiles PS50894 Histidine-containing phosphotransfer (HPt) domain profile.
798 885 InterPro IPR008207 Signal transduction histidine kinase, phosphotransfer (Hpt) domain
1 38 Phobius SIGNAL_PEPTIDE Signal peptide region
16 26 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
302 324 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
15 37 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
325 885 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GW72
AlphaFold — — full sequence — Viewing
ColabFold VK055_4884
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.906

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 9.29 0.498
2 3.07 0.102
3 2.75 0.083
4 2.65 0.078
5 1.74 0.031