Protein profile

VK055_4922

phosphocarrier, HPr family protein

Genome: KpATCC43816

Gene: AIK83448.1 Structure source: AlphaFold + ColabFold UniProt A0A0H3GSU9
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Amino acids 376
Annotations 5
Features 39
PDB binders 1
Druggability 0.733

Overview

Basic information about this protein and its source genome.

Accession
VK055_4922
Assembly
Klebsiella pneumoniae subsp. pneumoniae strain ATCC 43816 KPPR1, complete genome
Gene
AIK83448.1
Status
annotated
Amino acids
376
Structure source
AlphaFold + ColabFold
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule. GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport. GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Human identity (%)
0.0
Gut microbiome off-target
hit
Essential (DEG)
N
DEG identity (%)
49.153
Localization
Cytoplasmic
ColabFold pLDDT
92.04

Selected Druggability evidence

AlphaFold / UniProt model

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.733
Structure A0A0H3GSU9
Pocket Pocket 1
P2Rank 0.466
Structure A0A0H3GSU9
Pocket Pocket 1
ColabFold model
FPocket 0.713 · Pocket 1
P2Rank 0.327 · Pocket 1
Core conservation Conserved core gene
Roary core
CoreCruncher core
Gut microbiome 112 / 4744 genomes with a hit
Normalized 0.024

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

5 GO

Gene Ontology (GO)

5
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0016301 Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.
  • GO:0090563 Catalysis of the PEP-dependent, phosphoryl transfer-driven transport of substances across a membrane. The transport happens by catalysis of the reaction: protein S-phosphocysteine + sugar(out) = protein cysteine + sugar phosphate(in). This differs from primary and secondary active transport in that the solute is modified during transport.
  • GO:0009401 The uptake and phosphorylation of specific carbohydrates from the extracellular environment; uptake and phosphorylation are coupled, making the PTS a link between the uptake and metabolism of sugars; phosphoenolpyruvate is the original phosphate donor; phosphoenolpyruvate passes the phosphate via a signal transduction pathway, to enzyme 1 (E1), which in turn passes it on to the histidine protein, HPr; the next step in the system involves sugar-specific membrane-bound complex, enzyme 2 (EII), which transports the sugar into the cell; it includes the sugar permease, which catalyzes the transport reactions; EII is usually divided into three different domains, EIIA, EIIB, and EIIC.

Sequence Features

Domain/signature hits from InterPro and related databases.

39 records
Show feature table
Start End DB Term Name
287 371 FunFam G3DSA:3.30.1340.10:FF:000005 Fructose-specific PTS system IIA component
1 144 PANTHER PTHR30181 MANNITOL PERMEASE IIC COMPONENT
289 368 CDD cd00367 PTS-HPr_like
289 368 InterPro IPR000032 Phosphocarrier protein HPr-like
287 371 Gene3D G3DSA:3.30.1340.10 -
287 371 InterPro IPR035895 HPr-like superfamily
287 375 SUPERFAMILY SSF55594 HPr-like
287 375 InterPro IPR035895 HPr-like superfamily
286 368 NCBIfam TIGR01003 HPr family phosphocarrier protein
286 368 InterPro IPR000032 Phosphocarrier protein HPr-like
285 375 ProSiteProfiles PS51350 PTS HPR domain profile.
285 375 InterPro IPR000032 Phosphocarrier protein HPr-like
297 304 ProSitePatterns PS00369 PTS HPR domain histidine phosphorylation site signature.
297 304 InterPro IPR001020 Phosphotransferase system, HPr histidine phosphorylation site
325 340 PRINTS PR00107 Phosphocarrier protein signature
325 340 InterPro IPR000032 Phosphocarrier protein HPr-like
297 313 PRINTS PR00107 Phosphocarrier protein signature
297 313 InterPro IPR000032 Phosphocarrier protein HPr-like
340 357 PRINTS PR00107 Phosphocarrier protein signature
340 357 InterPro IPR000032 Phosphocarrier protein HPr-like
2 142 ProSiteProfiles PS51094 PTS_EIIA type-2 domain profile.
2 142 InterPro IPR002178 PTS EIIA type-2 domain
326 341 ProSitePatterns PS00589 PTS HPR domain serine phosphorylation site signature.
326 341 InterPro IPR002114 Phosphotransferase system, HPr serine phosphorylation site
5 139 Pfam PF00359 Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
5 139 InterPro IPR002178 PTS EIIA type-2 domain
2 140 SUPERFAMILY SSF55804 Phoshotransferase/anion transport protein
2 140 InterPro IPR016152 Phosphotransferase/anion transporter
1 142 FunFam G3DSA:3.40.930.10:FF:000006 Fructose-specific PTS system IIA component
150 276 SUPERFAMILY SSF55804 Phoshotransferase/anion transport protein
150 276 InterPro IPR016152 Phosphotransferase/anion transporter
4 139 CDD cd00211 PTS_IIA_fru
4 139 InterPro IPR002178 PTS EIIA type-2 domain
47 63 ProSitePatterns PS00372 PTS EIIA domains phosphorylation site signature 2.
47 63 InterPro IPR002178 PTS EIIA type-2 domain
1 142 Gene3D G3DSA:3.40.930.10 -
1 142 InterPro IPR016152 Phosphotransferase/anion transporter
287 369 Pfam PF00381 PTS HPr component phosphorylation site
287 369 InterPro IPR000032 Phosphocarrier protein HPr-like

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

0 + 2

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold AF_A0A0H3GSU9
AlphaFold — — full sequence — Viewing
ColabFold VK055_4922
ColabFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.733

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 4.31 0.178
2 4.08 0.164

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
PO3
2LRL
P0AA04 79.0 Da LogP -1.64 TPSA 63.2 ✓ Ro5 ✓ Clean [O-][P-](=O)[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.