KpATCC43816 Protein target profile

undecaprenyl-phosphate glucose phosphotransferase

Accession: VK055_5025

Gene: AIK83551.1 wcaJ 3D evidence: AlphaFold DB model + ColabFold model Metabolism 1 reaction UniProt A0A193SCR0
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Length 465
Pocket druggability (P2Rank · AlphaFold DB model) 0.731
Metabolic reactions 1
Chokepoint No
Functional annotation 0 EC 3 GO
Target summary

Promising target candidate with multiple supporting evidence streams.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

Independent pocket support: P2Rank + FPocket Both predictors report a high-scoring binding site (P2Rank 0.73, FPocket 0.90). Use the 3D pocket comparison to verify whether they identify the same site. No isoenzyme backup detected Imported GPR rules do not show an alternative enzyme for the catalyzed reaction set. No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Strong predicted binding pocket FPocket selected-pocket score 0.90. This is a computational pocket signal, not binding validation. Review the complete evidence below

Risks to review

Microbiome similarity 59 of 4744 screened genomes (1.2%) have a significant similarity hit. This target also has a predicted binding pocket (P2Rank 0.73) — check the ligand evidence before deprioritizing on off-target risk alone. Mixed conservation Core/accessory calls are not fully concordant.

Evidence gaps

No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome similarity
1.2% of screened genomes Lower prevalence suggests narrower overlap with the screened gut microbiome.

Essentiality

Essential (DEG)
N
DEG identity (%)
44.776 Higher values support similarity to known essential genes.

Structure confidence

ColabFold pLDDT
82.82 0-100 confidence; >70 supports local structural interpretation.

Binding-site evidence

AlphaFold DB / UniProt model

P2Rank's binding-site probability is the primary druggability signal shown across the app; FPocket's druggability score is shown alongside it for comparison. Both estimate small-molecule pocket quality after applying the curated structure priority — neither is experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

Druggability (P2Rank) 0.731
Structure A0A193SCR0
Pocket Pocket 1
Druggability (FPocket) 0.898
Structure A0A193SCR0
Pocket Pocket 15
ColabFold model
P2Rank 0.843 · Pocket 1
FPocket 0.928 · Pocket 11
Core conservation Accessory gene
Roary accessory
CoreCruncher accessory
Gut microbiome 59 / 4744 genomes with a hit
Prevalence 1.2%

Metabolic context

Reactions catalyzed, pathway membership, and centrality in the genome-scale metabolic network.

Explore metabolic network

Metabolic context: no human homolog detected.

Relative network centrality 0.0% more central than 0.0% of genes in this genome
Chokepoint Not a chokepoint
Pathways

No specific KEGG pathway assigned - this reaction either has no KEGG mapping, or only matches a generic overview map with no route-level information.

Catalyzed reaction

1 reaction mapped to this gene in the metabolic model. Open the full network to see each one, with substrates/products and the reaction-reaction map.

Imported from KpATCC43816.sbml · 2026-07-09

Sequence

Primary amino-acid sequence viewer.

MTISQHRFRSNANASIISMLQRFSDILIIFLGIYFSCFINDYFFNLHYVLMALVALVVFQMIGGITDFYRSWRGVEFSVELILILKNWSLSFLLTLGFVTLFSDFDLTFRTFIFWYLAVCAGFVVTRSLIRALAGFFRRIGYNKRRVAFAGSLPAGISLLETFRKQPWLGFEVKGIYEDSFSGTYDLELYAGKISDLINEARKGTIDRIYIAMHMRDEVAIKNMVSQLTDTTCSVLYIPDVFTFNILQSRTEEINGVPVVPLFDSPLNGINMVFKRLEDIIVSSLILILISPILLVIATAVKTTSKGPVIFRQVRYGMDGKPIKVWKFRSMTVMENDDKVIQATKNDIRVTKVGKFLRSTSLDELPQFFNVLFGQMSVVGPRPHAVSHNEQYRSLIQGYMLRHKVKPGITGLAQINGWRGETDTLEKMEKRIEYDLLYIRGWSIWLDLKIIFLTVFKGFINKSAY

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Subcellular localization

Localization
CytoplasmicMembrane

Gene Ontology (GO)

3
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0089702 Catalysis of the reaction: di-trans,octa-cis-undecaprenyl phosphate + UDP-alpha-D-glucose = alpha-D-glucosyl di-trans,octa-cis-undecaprenyl diphosphate + UMP.
  • GO:0009242 The chemical reactions and pathways resulting in the formation of colanic acid, a capsular bacterial polysaccharide.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
50 69 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
138 279 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
81 101 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
144 465 PANTHER PTHR30576 COLANIC BIOSYNTHESIS UDP-GLUCOSE LIPID CARRIER TRANSFERASE
275 457 Pfam PF02397 Bacterial sugar transferase
275 457 InterPro IPR003362 Bacterial sugar transferase
113 135 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
81 103 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
70 80 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
24 465 NCBIfam TIGR03023 undecaprenyl-phosphate glucose phosphotransferase
24 465 InterPro IPR017473 Undecaprenyl-phosphate glucose phosphotransferase, WcaJ
113 137 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
45 49 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
26 43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
26 44 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 25 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
102 112 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
280 301 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
24 465 NCBIfam TIGR03025 exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase
24 465 InterPro IPR017475 Exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase
302 465 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
142 263 Gene3D G3DSA:3.40.50.720 -
280 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
65 239 Pfam PF13727 CoA-binding domain
47 69 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
'Alpha spheres' is FPocket's own cavity-shape geometry, imported when available and aligned with the loaded structure.
'Pocket atoms'/'Predicted site atoms' show the pocket's residue atoms instead: P2Rank reports residues rather than alpha spheres, and FPocket falls back to this when alpha-sphere geometry is unavailable or doesn't align.
'No pocket geometry' means neither alpha spheres nor residue-position data could be found for that pocket; the layer just highlights the same residues as 'Nearby residues'.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · P2Rank

Druggability (P2Rank): high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Pocket 1 P2Rank #1
0.731
Likely same site as FPocket 15 1.1 Å 16 shared residues 100% of smaller site
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Surrounding area
Pocket 2 P2Rank #2
0.715
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Surrounding area
Pocket 3 P2Rank #3
0.11
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Surrounding area
Pocket 4 P2Rank #4
0.087
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Surrounding area
Pocket 5 P2Rank #5
0.065
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Surrounding area

Binding pockets · FPocket

Druggability (FPocket): high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Pocket 1 FPocket #15
0.898
Likely same site as P2Rank 1 1.1 Å 16 shared residues 100% of smaller site
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Surrounding area
Pocket 2 FPocket #21
0.23
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Surrounding area
All structural evidence 0 experimental · 2 predicted

Structural evidence

0 + 2

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB AF_A0A193SCR0
AlphaFold DB — — full sequence — Viewing
ColabFold VK055_5025
ColabFold — — full sequence — Loaded

Cross-references

External database identifiers for this protein, its structures, ligands, and metabolic reactions.

Sequence

UniProt A0A193SCR0

Pathways

BioCyc RXN-11791