Protein target profile

HT085_RS00015

polyphosphate kinase 1

Genome: NZ_AP023069.1 Gene: ppk1 TUM19854C_00030 E8M63_03535 ppk 3D evidence: AlphaFold DB model UniProt A0AAQ1DUC9
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Length 685
Functional annotation 1 EC 5 GO
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

Automated synthesis of the evidence currently loaded. Review the underlying records before prioritizing this protein.

Main supporting evidence

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Structure available AlphaFold DB model

Risks to review

No major risk signal surfaced in the loaded evidence.

Evidence gaps

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No FPocket pocket score No selected computational pocket-druggability value is available. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
Terms and data sources used on this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal Target pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Prioritization evidence

Selectivity, essentiality, structural confidence, conservation, and predicted binding-site evidence.

Off-target risk

Human off-target
No hit
Gut microbiome off-target
Hit

Essentiality

Essential (DEG)
Y

Localization

Localization
CytoplasmicMembrane

Binding-site evidence

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket Medium
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSSNLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQPELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFAVELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPGMNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRDFLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFDLVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALAGKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLKRYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPFTLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGMCTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIETATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDLLRG

Functional annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0008976 Catalysis of the reaction: ATP + phosphate(n) = ADP + phosphate(n+1).
  • GO:0009358 A protein complex that possesses polyphosphate kinase activity.
  • GO:0006799 The chemical reactions and pathways resulting in the formation of a polyphosphate, the anion or salt of polyphosphoric acid.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0046872 Binding to a metal ion.

Sequence domains and features

Domain and signature matches imported from InterPro and related databases.

28 records
Show feature table
Start End DB Term Name
113 301 Gene3D G3DSA:3.30.1840.10 Polyphosphate kinase middle domain
113 301 InterPro IPR036830 Polyphosphate kinase middle domain superfamily
3 682 PANTHER PTHR30218 POLYPHOSPHATE KINASE
3 682 InterPro IPR003414 Polyphosphate kinase
6 685 Hamap MF_00347 Polyphosphate kinase [ppk].
6 685 InterPro IPR003414 Polyphosphate kinase
9 112 Pfam PF13089 Polyphosphate kinase N-terminal domain
9 112 InterPro IPR025198 Polyphosphate kinase N-terminal domain
504 671 Pfam PF13090 Polyphosphate kinase C-terminal domain 2
504 671 InterPro IPR025200 Polyphosphate kinase C-terminal domain 2
332 494 Pfam PF17941 Polyphosphate kinase C-terminal domain 1
332 494 InterPro IPR041108 Polyphosphate kinase, C-terminal domain 1
8 677 NCBIfam TIGR03705 polyphosphate kinase 1
8 677 InterPro IPR003414 Polyphosphate kinase
1 685 PIRSF PIRSF015589 PPK_bact
1 685 InterPro IPR003414 Polyphosphate kinase
500 663 CDD cd09168 PLDc_PaPPK1_C2_like
319 501 Gene3D G3DSA:3.30.870.10 Endonuclease Chain A
9 112 Gene3D G3DSA:1.20.58.310 -
316 497 SUPERFAMILY SSF56024 Phospholipase D/nuclease
332 493 CDD cd09165 PLDc_PaPPK1_C1_like
110 315 SUPERFAMILY SSF143724 PHP14-like
502 685 Gene3D G3DSA:3.30.870.10 Endonuclease Chain A
122 321 Pfam PF02503 Polyphosphate kinase middle domain
122 321 InterPro IPR024953 Polyphosphate kinase middle domain
4 107 SUPERFAMILY SSF140356 PPK N-terminal domain-like
4 107 InterPro IPR036832 Polyphosphate kinase N-terminal domain superfamily
502 683 SUPERFAMILY SSF56024 Phospholipase D/nuclease

3D structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

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Pocket score High Medium Low
How colors and pocket overlays are used
Uniform protein color marks the displayed model as a single molecular object.
Experimental PDB structures may be colored by chain to distinguish subunits or copies present in the file.
Pocket colors and alpha spheres are evidence overlays for predicted binding cavities; they are not alternative protein chains.
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #7
0.611
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Surrounding area
Site 2 FPocket #6
0.236
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Surrounding area

Binding pockets · P2Rank

Probability: high ≥ 0.5 · medium 0.2–0.49 · low < 0.2

Site 1 P2Rank #1
0.741
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Surrounding area
Site 2 P2Rank #2
0.531
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Surrounding area
Site 3 P2Rank #3
0.351
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Surrounding area
Site 4 P2Rank #4
0.309
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Surrounding area
Site 5 P2Rank #5
0.303
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Surrounding area
All structural evidence 0 experimental · 1 predicted

Structural evidence

0 + 1

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB HT085_RS00015
AlphaFold DB — — full sequence — Viewing