Protein profile

PA0008

glycine--tRNA ligase subunit beta

Genome: NC_002516.2

Gene: PA0008 glyS Structure source: AlphaFold UniProt Q9I7B8
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Amino acids 684
Annotations 9
Features 25
PDB binders 0
Druggability 0.812

Overview

Basic information about this protein and its source genome.

Accession
PA0008
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0008 glyS
Status
annotated
Amino acids
684
Structure source
AlphaFold
EC
6.1.1.14
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0004814 Catalysis of the reaction: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0004820 Catalysis of the reaction: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). GO:0006420 The process of coupling arginine to arginyl-tRNA, catalyzed by arginyl-tRNA synthetase. The arginyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of an alanine accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification. GO:0006426 The process of coupling glycine to glycyl-tRNA, catalyzed by glycyl-tRNA synthetase. The glycyll-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a glycine-accepting tRNA.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.812
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0004814 Catalysis of the reaction: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0004820 Catalysis of the reaction: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).
  • GO:0006420 The process of coupling arginine to arginyl-tRNA, catalyzed by arginyl-tRNA synthetase. The arginyl-tRNA synthetase is a class-I synthetase. The activated amino acid is transferred to the 2'-OH group of an alanine accetping tRNA. The 2'-O-aminoacyl-tRNA will ultimately migrate to the 3' position via transesterification.
  • GO:0006426 The process of coupling glycine to glycyl-tRNA, catalyzed by glycyl-tRNA synthetase. The glycyll-tRNA synthetase is a class-II synthetase. The activated amino acid is transferred to the 3'-OH group of a glycine-accepting tRNA.
  • GO:0000166 Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records
Show feature table
Start End DB Term Name
334 485 SUPERFAMILY SSF109604 HD-domain/PDEase-like
5 683 NCBIfam TIGR00211 glycine--tRNA ligase subunit beta
5 683 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
6 544 Pfam PF02092 Glycyl-tRNA synthetase beta subunit
6 544 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
3 683 Hamap MF_00255 Glycine--tRNA ligase beta subunit [glyS].
3 683 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
358 648 ProSiteProfiles PS50861 Heterodimeric glycyl-transfer RNA synthetases family profile.
358 648 InterPro IPR006194 Glycine-tRNA synthetase, heterodimeric
577 671 Pfam PF05746 DALR anticodon binding domain
577 671 InterPro IPR008909 DALR anticodon binding
3 683 PANTHER PTHR30075 GLYCYL-TRNA SYNTHETASE
3 683 InterPro IPR006194 Glycine-tRNA synthetase, heterodimeric
580 683 SMART SM00836 dalr_1_4
580 683 InterPro IPR008909 DALR anticodon binding
241 256 PRINTS PR01045 Glycyl-tRNA synthetase beta subunit signature
241 256 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
313 329 PRINTS PR01045 Glycyl-tRNA synthetase beta subunit signature
313 329 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
7 20 PRINTS PR01045 Glycyl-tRNA synthetase beta subunit signature
7 20 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
389 408 PRINTS PR01045 Glycyl-tRNA synthetase beta subunit signature
389 408 InterPro IPR015944 Glycine-tRNA ligase, beta subunit
50 62 PRINTS PR01045 Glycyl-tRNA synthetase beta subunit signature
50 62 InterPro IPR015944 Glycine-tRNA ligase, beta subunit

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0008
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
5 0.812
2 0.583
1 0.361
12 0.202