Protein profile

PA0280

sulfate.thiosulfate ABC transporter ATP-binding protein CysA

Genome: NC_002516.2

Gene: cysA PA0280 Structure source: AlphaFold UniProt Q9I6L0
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Amino acids 329
Annotations 9
Features 22
PDB binders 6
Druggability 0.823

Overview

Basic information about this protein and its source genome.

Accession
PA0280
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
cysA PA0280
Status
annotated
Amino acids
329
Structure source
AlphaFold
EC
7.3.2.3
GO
GO:0043190 A complex for the transport of metabolites into and out of the cell, typically comprised of four domains; two membrane-associated domains and two ATP-binding domains at the intracellular face of the membrane, that form a central pore through the plasma membrane. Each of the four core domains may be encoded as a separate polypeptide or the domains can be fused in any one of a number of ways into multidomain polypeptides. In Bacteria and Archaebacteria, ABC transporters also include substrate binding proteins to bind substrate external to the cytoplasm and deliver it to the transporter. GO:0015419 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + sulfate(out) = ADP + phosphate + sulfate(in). GO:0102025 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + thiosulfate(out) = ADP + phosphate + thiosulfate(in). GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. GO:1902358 The directed movement of sulfate across a membrane.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
58.974
Human E-value
1.45e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.823
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0043190 A complex for the transport of metabolites into and out of the cell, typically comprised of four domains; two membrane-associated domains and two ATP-binding domains at the intracellular face of the membrane, that form a central pore through the plasma membrane. Each of the four core domains may be encoded as a separate polypeptide or the domains can be fused in any one of a number of ways into multidomain polypeptides. In Bacteria and Archaebacteria, ABC transporters also include substrate binding proteins to bind substrate external to the cytoplasm and deliver it to the transporter.
  • GO:0015419 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + sulfate(out) = ADP + phosphate + sulfate(in).
  • GO:0102025 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: ATP + H2O + thiosulfate(out) = ADP + phosphate + thiosulfate(in).
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
  • GO:1902358 The directed movement of sulfate across a membrane.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0008272 OBSOLETE. The directed movement of sulfate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records
Show feature table
Start End DB Term Name
18 164 Pfam PF00005 ABC transporter
18 164 InterPro IPR003439 ABC transporter-like, ATP-binding domain
3 237 ProSiteProfiles PS50893 ATP-binding cassette, ABC transporter-type domain profile.
3 237 InterPro IPR003439 ABC transporter-like, ATP-binding domain
137 151 ProSitePatterns PS00211 ABC transporters family signature.
137 151 InterPro IPR017871 ABC transporter-like, conserved site
2 245 PANTHER PTHR42781 SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA
3 243 NCBIfam TIGR00968 sulfate ABC transporter ATP-binding protein
3 243 InterPro IPR005666 Sulphate transport system permease protein 1
2 278 Gene3D G3DSA:3.40.50.300 -
2 278 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
2 251 FunFam G3DSA:3.40.50.300:FF:000227 Sulfate/thiosulfate import ATP-binding protein CysA
235 324 SUPERFAMILY SSF50331 MOP-like
235 324 InterPro IPR008995 Molybdate/tungstate binding, C-terminal
1 239 CDD cd03296 ABC_CysA_sulfate_importer
1 239 InterPro IPR005666 Sulphate transport system permease protein 1
269 321 Pfam PF12857 TOBE-like domain
269 321 InterPro IPR024765 TOBE-like domain
3 243 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
3 243 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
27 214 SMART SM00382 AAA_5
27 214 InterPro IPR003593 AAA+ ATPase domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0280
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.823
5 0.265

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

56 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
2BA
7AHE
Q9KIF7 658.4 Da LogP -1.63 TPSA 309.7 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@H]4[C@H](O3)C…
ANP
1OXV
Q97UY8 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
BET
7AHH
Q9KIF7 118.2 Da LogP -0.22 TPSA 37.3 ✓ Ro5 ✓ Clean C[N+](C)(C)CC(=O)O
NH4
1G29
Q9YGA6 18.0 Da LogP 0.38 TPSA 36.5 ✓ Ro5 ✓ Clean [NH4+]
POP
2D62
O57933 176.0 Da LogP -2.08 TPSA 129.9 ✓ Ro5 ✓ Clean O[P@@](=O)([O-])O[P@@](=O)(O)[O-]
VO4
7D0A
A0A086HZU3 114.9 Da LogP -3.69 TPSA 86.2 ✓ Ro5 ✓ Clean [O-][V](=O)([O-])[O-]

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.