Protein profile

PA0381

thiazole synthase

Genome: NC_002516.2

Gene: thiG PA0381 Structure source: Experimental + AlphaFold UniProt Q9I6B4
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Amino acids 265
Annotations 6
Features 11
PDB binders 0
Druggability 0.445

Overview

Basic information about this protein and its source genome.

Accession
PA0381
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
thiG PA0381
Status
annotated
Amino acids
265
Structure source
Experimental + AlphaFold
EC
2.8.1.10
GO
GO:1902508 A protein complex which is capable of 2-iminoacetate synthase activity. GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:1990107 Catalysis of the reaction: 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H+ + 2 H2O. GO:0009228 The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver. GO:0009229 The chemical reactions and pathways resulting in the formation of thiamine diphosphate, a derivative of thiamine (vitamin B1) which acts as a coenzyme in a range of processes including the Krebs cycle.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.445
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:1902508 A protein complex which is capable of 2-iminoacetate synthase activity.
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:1990107 Catalysis of the reaction: 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H+ + 2 H2O.
  • GO:0009228 The chemical reactions and pathways resulting in the formation of thiamine (vitamin B1), a water soluble vitamin present in fresh vegetables and meats, especially liver.
  • GO:0009229 The chemical reactions and pathways resulting in the formation of thiamine diphosphate, a derivative of thiamine (vitamin B1) which acts as a coenzyme in a range of processes including the Krebs cycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

11 records
Show feature table
Start End DB Term Name
5 264 PANTHER PTHR34266 THIAZOLE SYNTHASE
5 264 InterPro IPR008867 Thiazole synthase
11 260 CDD cd04728 ThiG
11 260 InterPro IPR008867 Thiazole synthase
13 259 Pfam PF05690 Thiazole biosynthesis protein ThiG
13 259 InterPro IPR033983 Thiazole synthase ThiG
1 265 Gene3D G3DSA:3.20.20.70 Aldolase class I
1 265 InterPro IPR013785 Aldolase-type TIM barrel
10 251 SUPERFAMILY SSF110399 ThiG-like
10 265 Hamap MF_00443 Thiazole synthase [thiG].
10 265 InterPro IPR008867 Thiazole synthase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 1WV2
X-ray 2.90 Å A,B
100.0% 1-265
PDBe RCSB PDBj File
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AlphaFold PA0381
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.445
2 0.317

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 10.97 0.588
2 1.87 0.037