Protein profile

PA0382

tRNA (guanine-N(7)-)-methyltransferase

Genome: NC_002516.2

Gene: micA Structure source: ColabFold
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Amino acids 223
Annotations 2
Features 16
PDB binders 0
Druggability 0.596

Overview

Basic information about this protein and its source genome.

Accession
PA0382
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
micA
Status
annotated
Amino acids
223
Structure source
ColabFold
GO
GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically. GO:0008176 Catalysis of the reaction: guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
26.271
Human E-value
3.51e-07
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.596
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

2 GO

Gene Ontology (GO)

2
  • GO:0006400 The covalent alteration of one or more nucleotides within a tRNA molecule to produce a tRNA molecule with a sequence that differs from that coded genetically.
  • GO:0008176 Catalysis of the reaction: guanosine46 in tRNA + S-adenosyl-L-methionine = N7-methylguanosine46 in tRNA + S-adenosyl-L-homocysteine.

Sequence Features

Domain/signature hits from InterPro and related databases.

16 records
Show feature table
Start End DB Term Name
5 221 ProSiteProfiles PS51625 SAM-dependent methyltransferase TRMB-type domain profile.
5 221 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
53 161 CDD cd02440 AdoMet_MTases
14 223 FunFam G3DSA:3.40.50.150:FF:000024 tRNA (guanine-N(7)-)-methyltransferase
35 221 PANTHER PTHR23417 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE
35 221 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
2 221 SUPERFAMILY SSF53335 S-adenosyl-L-methionine-dependent methyltransferases
2 221 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
14 223 Gene3D G3DSA:3.40.50.150 Vaccinia Virus protein VP39
14 223 InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase superfamily
34 222 NCBIfam TIGR00091 tRNA (guanosine(46)-N7)-methyltransferase TrmB
34 222 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
8 222 Hamap MF_01057 tRNA (guanine-N(7)-)-methyltransferase [trmB].
8 222 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type
48 217 Pfam PF02390 Putative methyltransferase
48 217 InterPro IPR003358 tRNA (guanine-N-7) methyltransferase, Trmb type

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
ColabFold PA0382
ColabFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.596
1 0.515
3 0.377