Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA0386
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA0386
Status: annotated
Amino acids: 384
Structure source: AlphaFold

GO

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0004109 Catalysis of the reaction: coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX. GO:0046872 Binding to a metal ion. GO:0006779 The chemical reactions and pathways resulting in the formation of any member of a large group of derivatives or analogs of porphyrin. Porphyrin consists of a ring of four pyrrole nuclei linked each to the next at their alpha positions through a methine group. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target: hit
Human identity (%): 36.842
Human E-value: 5.800000000000001e-56
Gut microbiome off-target: hit
Essential (DEG): N
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.581

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO:0004109 Catalysis of the reaction: coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX.
GO:0046872 Binding to a metal ion.
GO:0006779 The chemical reactions and pathways resulting in the formation of any member of a large group of derivatives or analogs of porphyrin. Porphyrin consists of a ring of four pyrrole nuclei linked each to the next at their alpha positions through a methine group.
GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0051536 Binding to an iron-sulfur cluster, a combination of iron and sulfur atoms.

Sequence Features

Domain/signature hits from InterPro and related databases.

22 records

Show feature table

Start	End	DB	Term	Name
6	242	ProSiteProfiles	PS51918	Radical SAM core domain profile.
6	242	InterPro	IPR007197	Radical SAM
11	227	SMART	SM00729	MiaB
11	227	InterPro	IPR006638	Elp3/MiaA/NifB-like, radical SAM core domain
10	383	SUPERFAMILY	SSF102114	Radical SAM enzymes
13	346	NCBIfam	TIGR00539	radical SAM family heme chaperone HemW
13	346	InterPro	IPR004559	Heme chaperone HemW-like
16	183	Pfam	PF04055	Radical SAM superfamily
16	183	InterPro	IPR007197	Radical SAM
20	222	CDD	cd01335	Radical_SAM
21	252	SFLD	SFLDG01082	B12-binding domain containing
7	383	SFLD	SFLDF00288	HemN-like, clustered with nucleoside-triphosphate RdgB
7	383	SFLD	SFLDS00029	Radical SAM
7	383	InterPro	IPR007197	Radical SAM
10	383	PANTHER	PTHR13932	COPROPORPHYRINIGEN III OXIDASE
10	383	InterPro	IPR034505	Anaerobic coproporphyrinogen-III oxidase
11	383	SFLD	SFLDF00562	HemN-like, clustered with heat shock genes
11	383	InterPro	IPR004559	Heme chaperone HemW-like
14	223	Gene3D	G3DSA:3.20.20.70	Aldolase class I
14	223	InterPro	IPR013785	Aldolase-type TIM barrel
309	373	Pfam	PF06969	HemN C-terminal domain
309	373	InterPro	IPR010723	HemN, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA0386	AlphaFold	—	—	full sequence	—	Viewing

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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.581

PA0386