Protein profile

PA0395

twitching motility protein PilT

Genome: NC_002516.2

Gene: PA0395 pilT Structure source: Experimental + AlphaFold UniProt P24559
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Amino acids 344
Annotations 6
Features 18
PDB binders 3
Druggability 0.61

Overview

Basic information about this protein and its source genome.

Accession
PA0395
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0395 pilT
Status
annotated
Amino acids
344
Structure source
Experimental + AlphaFold
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. GO:0043108 The process of withdrawing a pilus back into a cell. GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.61
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

6
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0044096 A short filamentous structure on the surface of a bacterial cell distinguished from other pili by post-translational N-methylation of the pilin monomers.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0016887 Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
  • GO:0043108 The process of withdrawing a pilus back into a cell.
  • GO:0043107 Any process involved in the controlled movement of a bacterial cell which is dependent on the presence of type IV pili. Includes social gliding motility and twitching motility.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records
Show feature table
Start End DB Term Name
1 100 Gene3D G3DSA:3.30.450.90 -
101 344 FunFam G3DSA:3.40.50.300:FF:000872 Twitching motility protein PilT
193 207 ProSitePatterns PS00662 Bacterial type II secretion system protein E signature.
193 207 InterPro IPR001482 Type II/IV secretion system domain
3 342 NCBIfam TIGR01420 PilT/PilU family type 4a pilus ATPase
3 342 InterPro IPR006321 Pilus retraction protein PilT/PilU
103 324 CDD cd01131 PilT
103 324 InterPro IPR006321 Pilus retraction protein PilT/PilU
101 344 Gene3D G3DSA:3.40.50.300 -
101 344 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
122 256 SMART SM00382 AAA_5
122 256 InterPro IPR003593 AAA+ ATPase domain
10 338 PANTHER PTHR30486 TWITCHING MOTILITY PROTEIN PILT
1 100 FunFam G3DSA:3.30.450.90:FF:000002 Twitching motility protein PilT
5 335 SUPERFAMILY SSF52540 P-loop containing nucleoside triphosphate hydrolases
5 335 InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
11 271 Pfam PF00437 Type II/IV secretion system protein
11 271 InterPro IPR001482 Type II/IV secretion system domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

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Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 3JVV
X-ray 2.60 Å A,B,C
100.0% 1-344
PDBe RCSB PDBj File
Viewing
PDB 3JVU
X-ray 3.10 Å A,B,C
100.0% 1-344
PDBe RCSB PDBj File
Loaded
AlphaFold PA0395
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.61
1 0.401

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.27 0.114
2 2.8 0.086
3 2.23 0.054
4 1.73 0.031

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
ACP
3JVV 2.60 Å Open crystal
505.2 Da LogP -1.52 TPSA 269.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.