Protein profile

PA0407

glutathione synthetase

Genome: NC_002516.2

Gene: PA0407 gshB Structure source: AlphaFold UniProt Q9I697
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Amino acids 317
Annotations 6
Features 26
PDB binders 1
Druggability 0.608

Overview

Basic information about this protein and its source genome.

Accession
PA0407
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0407 gshB
Status
annotated
Amino acids
317
Structure source
AlphaFold
EC
6.3.2.3
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0004363 Catalysis of the reaction: L-gamma-glutamyl-L-cysteine + ATP + glycine = ADP + glutathione + 2 H+ + phosphate. GO:0046872 Binding to a metal ion. GO:0006750 The chemical reactions and pathways resulting in the formation of glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
26.295
Human E-value
2.65e-06
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.608
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0004363 Catalysis of the reaction: L-gamma-glutamyl-L-cysteine + ATP + glycine = ADP + glutathione + 2 H+ + phosphate.
  • GO:0046872 Binding to a metal ion.
  • GO:0006750 The chemical reactions and pathways resulting in the formation of glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

26 records
Show feature table
Start End DB Term Name
138 202 FunFam G3DSA:3.30.1490.20:FF:000009 Glutathione synthetase
125 297 Pfam PF02955 Prokaryotic glutathione synthetase, ATP-grasp domain
125 297 InterPro IPR004218 Prokaryotic glutathione synthetase, ATP-binding
188 301 FunFam G3DSA:3.30.470.20:FF:000010 Glutathione synthetase
1 20 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
124 311 SUPERFAMILY SSF56059 Glutathione synthetase ATP-binding domain-like
34 317 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
4 121 Pfam PF02951 Prokaryotic glutathione synthetase, N-terminal domain
4 121 InterPro IPR004215 Prokaryotic glutathione synthetase, N-terminal
1 317 Hamap MF_00162 Glutathione synthetase [gshB].
1 317 InterPro IPR006284 Glutathione synthetase, prokaryotic
29 33 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
21 28 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
8 116 FunFam G3DSA:3.40.50.20:FF:000009 Glutathione synthetase
1 314 PANTHER PTHR21621 RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN
3 123 SUPERFAMILY SSF52440 PreATP-grasp domain
3 123 InterPro IPR016185 Pre-ATP-grasp domain superfamily
116 301 Gene3D G3DSA:3.30.470.20 -
4 313 NCBIfam TIGR01380 glutathione synthase
4 313 InterPro IPR006284 Glutathione synthetase, prokaryotic
138 202 Gene3D G3DSA:3.30.1490.20 -
138 202 InterPro IPR013815 ATP-grasp fold, subdomain 1
1 33 Phobius SIGNAL_PEPTIDE Signal peptide region
126 311 ProSiteProfiles PS50975 ATP-grasp fold profile.
126 311 InterPro IPR011761 ATP-grasp fold
8 302 Gene3D G3DSA:3.40.50.20 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0407
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.608
2 0.448

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

51 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
GSH
1GSA
P04425 307.3 Da LogP -2.21 TPSA 158.8 1 viol. ✓ Clean C(CC(=O)N[C@@H](CS)C(=O)NCC(=O)O)[C@@H](C(=O)O)N

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.