Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA0425
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: mexA PA0425
Status: annotated
Amino acids: 383
Structure source: Experimental + AlphaFold

GO

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015562 Enables the transfer of a specific substance or related group of substances from the inside of the cell to the outside of the cell across a membrane. GO:0042802 Binding to an identical protein or proteins. GO:0051260 The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer. GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms. GO:0009636 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.392

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

10 GO

Gene Ontology (GO)

10

GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0015562 Enables the transfer of a specific substance or related group of substances from the inside of the cell to the outside of the cell across a membrane.
GO:0042802 Binding to an identical protein or proteins.
GO:0051260 The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
GO:0046677 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an antibiotic stimulus. An antibiotic is a chemical substance produced by a microorganism which has the capacity to inhibit the growth of or to kill other microorganisms.
GO:0009636 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a toxic stimulus.
GO:0042908 The directed movement of a xenobiotic into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. A xenobiotic is a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

24 records

Show feature table

Start	End	DB	Term	Name
1	22	Phobius	SIGNAL_PEPTIDE	Signal peptide region
188	269	FunFam	G3DSA:2.40.30.170:FF:000001	Multidrug resistance efflux transporter MdtE
23	383	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
56	268	Gene3D	G3DSA:2.40.30.170	-
97	156	Gene3D	G3DSA:1.10.287.470	Helix hairpin bin
1	24	ProSiteProfiles	PS51257	Prokaryotic membrane lipoprotein lipid attachment site profile.
1	8	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
269	362	Gene3D	G3DSA:2.40.420.20	-
1	29	SignalP_EUK	SignalP-noTM	SignalP-noTM
10	373	PANTHER	PTHR30158	ACRA/E-RELATED COMPONENT OF DRUG EFFLUX TRANSPORTER
19	22	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
59	278	Pfam	PF16576	Barrel-sandwich domain of CusB or HlyD membrane-fusion
59	278	InterPro	IPR032317	RND efflux pump, membrane fusion protein, barrel-sandwich domain
49	285	SUPERFAMILY	SSF111369	HlyD-like secretion proteins
62	192	Gene3D	G3DSA:2.40.50.100	-
1	29	SignalP_GRAM_POSITIVE	SignalP-TM	SignalP-TM
279	346	Pfam	PF00529	Cation efflux system protein CusB domain 1
279	346	InterPro	IPR043602	Cation efflux system protein CusB, domain 1
9	18	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.
269	361	FunFam	G3DSA:2.40.420.20:FF:000001	Efflux RND transporter periplasmic adaptor subunit
1	22	SignalP_GRAM_NEGATIVE	SignalP-noTM	SignalP-noTM
38	360	NCBIfam	TIGR01730	efflux RND transporter periplasmic adaptor subunit
38	360	InterPro	IPR006143	RND efflux pump, membrane fusion protein
103	123	Coils	Coil	Coil

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

4 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
PDB 1T5E	X-ray	3.00 Å	A,B,C,D,E,F,G,H,I,J,K,L,M	94.0% 24-383	PDBe RCSB PDBj File	Viewing
PDB 2V4D	X-ray	3.20 Å	A,B,C,D,E,F,G,H,I,J,K,L,M	94.0% 24-383	PDBe RCSB PDBj File	Loaded
PDB 1VF7	X-ray	2.40 Å	A,B,C,D,E,F,G,H,I,J,K,L,M	93.7% 25-383	PDBe RCSB PDBj File	Loaded
PDB 4DK1	X-ray	3.50 Å	A,B,C,D	16.7% 95-158	PDBe RCSB PDBj File	Loaded
AlphaFold PA0425	AlphaFold	—	—	full sequence	—	Loaded

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
6				0.804
1				0.369

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Ligand	Source crystal	MW · LogP · TPSA	Lipinski	PAINS	SMILES
3GR	1T5E 3.00 Å Open crystal	90.1 Da LogP -1.46 TPSA 57.5	✓ Ro5	✓ Clean	`C([C@H](C=O)O)O`

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

No PDB ligands found through similar proteins.

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

No virtual-screening candidates for this protein.

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.

PA0425