Protein profile

PA0458

major facilitator superfamily transporter

Genome: NC_002516.2

Gene: PA0458 Structure source: AlphaFold UniProt Q9I660
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Amino acids 477
Annotations 4
Features 61
PDB binders 1
Druggability 0.899

Overview

Basic information about this protein and its source genome.

Accession
PA0458
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0458
Status
annotated
Amino acids
477
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.899
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Sequence Features

Domain/signature hits from InterPro and related databases.

61 records
Show feature table
Start End DB Term Name
15 462 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
15 462 InterPro IPR020846 Major facilitator superfamily domain
1 14 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
459 477 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
260 464 FunFam G3DSA:1.20.1250.20:FF:000021 Putative multidrug resistance protein MdtD
386 396 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
438 458 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
356 378 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
17 36 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
436 458 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
295 317 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
106 128 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
81 100 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
202 219 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
167 189 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
20 410 Pfam PF07690 Major Facilitator Superfamily
20 410 InterPro IPR011701 Major facilitator superfamily
38 48 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
304 322 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
262 465 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
262 465 InterPro IPR036259 MFS transporter superfamily
323 333 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
16 247 Gene3D G3DSA:1.20.1720.10 Multidrug resistance protein D
229 248 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
70 80 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
230 248 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
353 357 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
49 69 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
329 351 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
140 162 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
268 292 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
268 290 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
101 105 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
19 417 NCBIfam TIGR00711 DHA2 family efflux MFS transporter permease subunit
19 417 InterPro IPR004638 Drug resistance transporter EmrB-like
128 138 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
249 267 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
16 226 FunFam G3DSA:1.20.1720.10:FF:000001 Putative multidrug resistance protein MdtD
139 161 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
419 437 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
399 421 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
189 199 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
162 166 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
358 385 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
80 102 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
167 188 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
219 229 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
51 73 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
4 467 SUPERFAMILY SSF103473 MFS general substrate transporter
4 467 InterPro IPR036259 MFS transporter superfamily
200 218 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
109 129 PRINTS PR01036 Tetracycline resistance protein TetB signature
18 42 PRINTS PR01036 Tetracycline resistance protein TetB signature
139 163 PRINTS PR01036 Tetracycline resistance protein TetB signature
15 37 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
19 457 CDD cd17503 MFS_LmrB_MDR_like
293 303 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
334 352 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
17 468 PANTHER PTHR42718 MAJOR FACILITATOR SUPERFAMILY MULTIDRUG TRANSPORTER MFSC
106 127 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
397 418 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0458
AlphaFold — — full sequence — Viewing
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.899
10 0.318

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

144 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
J0M
6E9O
J7QAK3 196.2 Da LogP -3.49 TPSA 138.5 1 viol. ✓ Clean C([C@H]([C@@H]([C@@H]([C@H](C(=O)O)O)O)O)O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.