Protein profile

PA0600

two-component sensor

Genome: NC_002516.2

Gene: PA0600 Structure source: AlphaFold UniProt Q9I5T8
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Amino acids 797
Annotations 13
Features 70
PDB binders 3
Druggability 0.638

Overview

Basic information about this protein and its source genome.

Accession
PA0600
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0600
Status
annotated
Amino acids
797
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0046872 Binding to a metal ion. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.638
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 12 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

12
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0046872 Binding to a metal ion.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0046983 The formation of a protein dimer, a macromolecular structure consists of two noncovalently associated identical or nonidentical subunits.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0005515 Binding to a protein.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

70 records
Show feature table
Start End DB Term Name
126 221 Gene3D G3DSA:3.40.190.10 -
527 568 SMART SM00086 pac_2
527 568 InterPro IPR001610 PAC motif
396 436 SMART SM00086 pac_2
396 436 InterPro IPR001610 PAC motif
474 565 CDD cd00130 PAS
474 565 InterPro IPR000014 PAS domain
5 16 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
476 560 Pfam PF08447 PAS fold
476 560 InterPro IPR013655 PAS fold-3
21 271 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
596 662 Pfam PF07730 Histidine kinase
596 662 InterPro IPR011712 Signal transduction histidine kinase, subgroup 3, dimerisation and phosphoacceptor domain
665 792 Gene3D G3DSA:3.30.565.10 -
665 792 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
272 293 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
395 445 ProSiteProfiles PS50113 PAC domain profile.
395 445 InterPro IPR000700 PAS-associated, C-terminal
433 566 SUPERFAMILY SSF55785 PYP-like sensor domain (PAS domain)
433 566 InterPro IPR035965 PAS domain superfamily
700 794 SMART SM00387 HKATPase_4
700 794 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
567 655 Gene3D G3DSA:1.20.5.1930 -
446 523 ProSiteProfiles PS50112 PAS repeat profile.
446 523 InterPro IPR000014 PAS domain
335 445 SUPERFAMILY SSF55785 PYP-like sensor domain (PAS domain)
335 445 InterPro IPR035965 PAS domain superfamily
17 20 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 20 Phobius SIGNAL_PEPTIDE Signal peptide region
323 389 SMART SM00091 pas_2
323 389 InterPro IPR000014 PAS domain
448 521 SMART SM00091 pas_2
448 521 InterPro IPR000014 PAS domain
778 791 PRINTS PR00344 Bacterial sensor protein C-terminal signature
778 791 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
754 772 PRINTS PR00344 Bacterial sensor protein C-terminal signature
754 772 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
736 750 PRINTS PR00344 Bacterial sensor protein C-terminal signature
736 750 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
33 258 Gene3D G3DSA:3.40.190.10 -
41 262 SMART SM00062 AABind_6
41 262 InterPro IPR001638 Solute-binding protein family 3/N-terminal domain of MltF
330 438 Pfam PF08448 PAS fold
330 438 InterPro IPR013656 PAS fold-4
474 575 NCBIfam TIGR00229 PAS domain S-box protein
474 575 InterPro IPR000014 PAS domain
329 443 NCBIfam TIGR00229 PAS domain S-box protein
329 443 InterPro IPR000014 PAS domain
39 259 CDD cd01007 PBP2_BvgS_HisK_like
1 20 SignalP_EUK SignalP-noTM SignalP-noTM
702 792 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
702 792 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
37 262 SUPERFAMILY SSF53850 Periplasmic binding protein-like II
705 791 CDD cd16917 HATPase_UhpB-NarQ-NarX-like
1 20 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
42 260 Pfam PF00497 Bacterial extracellular solute-binding proteins, family 3
42 260 InterPro IPR001638 Solute-binding protein family 3/N-terminal domain of MltF
671 792 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
671 792 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
436 456 Coils Coil Coil
323 450 Gene3D G3DSA:3.30.450.20 PAS domain
342 433 CDD cd00130 PAS
342 433 InterPro IPR000014 PAS domain
1 4 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
294 797 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
599 794 ProSiteProfiles PS50109 Histidine kinase domain profile.
599 794 InterPro IPR005467 Histidine kinase domain
451 566 Gene3D G3DSA:3.30.450.20 PAS domain
138 795 PANTHER PTHR24421 NITRATE/NITRITE SENSOR PROTEIN NARX-RELATED
568 595 Coils Coil Coil

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0600
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.638
5 0.607

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ACP
3EHJ
O34757 505.2 Da LogP -1.52 TPSA 269.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
KH2
5KKW
Q4FLR5 221.3 Da LogP 0.55 TPSA 57.2 ✓ Ro5 ✓ Clean C[N+]1(CCCCC1)CCCS(=O)(=O)[O-]
PG5
3MR0
Q2T6J3 178.2 Da LogP 0.31 TPSA 36.9 ✓ Ro5 ✓ Clean COCCOCCOCCOC

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.