Protein profile

PA0794

aconitate hydratase

Genome: NC_002516.2

Gene: PA0794 Structure source: AlphaFold UniProt Q9I5E4
Amino acids 868
Annotations 8
Features 38
PDB binders 11
Druggability 0.575

Overview

Basic information about this protein and its source genome.

Accession
PA0794
Gene
PA0794
Status
annotated
Amino acids
868
Structure source
AlphaFold

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
48.101
Human E-value
1.29e-18
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.575
Structure
Pocket

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
  • GO:0003994 Catalysis of the reaction: citrate = isocitrate. The reaction occurs in two steps: (1) citrate = cis-aconitate + H2O, (2) cis-aconitate + H2O = isocitrate. This reaction is the interconversion of citrate and isocitrate via the labile, enzyme-bound intermediate cis-aconitate. Water is removed from one part of the citrate molecule and added back to a different atom to form isocitrate.
  • GO:0030350 Binding to an iron-responsive element, a regulatory sequence found in the 5'- and 3'-untranslated regions of mRNAs encoding many iron-binding proteins.
  • GO:0046872 Binding to a metal ion.
  • GO:0019679 OBSOLETE. The chemical reactions and pathways involving propionate that occur in the methylcitrate cycle.
  • GO:0006099 A nearly universal metabolic pathway in which the acetyl group of acetyl coenzyme A is effectively oxidized to two CO2 and four pairs of electrons are transferred to coenzymes. The acetyl group combines with oxaloacetate to form citrate, which undergoes successive transformations to isocitrate, 2-oxoglutarate, succinyl-CoA, succinate, fumarate, malate, and oxaloacetate again, thus completing the cycle. In eukaryotes the tricarboxylic acid is confined to the mitochondria. See also glyoxylate cycle.

Sequence Features

Domain/signature hits from InterPro and related databases.

38 records
Show feature table
Start End DB Term Name
566 622 Gene3D G3DSA:6.10.190.10 -
661 792 Pfam PF00694 Aconitase C-terminal domain
661 792 InterPro IPR000573 Aconitase A/isopropylmalate dehydratase small subunit, swivel domain
2 864 NCBIfam TIGR02333 Fe/S-dependent 2-methylisocitrate dehydratase AcnD
2 864 InterPro IPR012708 2-methylisocitrate dehydratase AcnD, Fe/S-dependent
603 862 SUPERFAMILY SSF52016 LeuD/IlvD-like
23 591 SUPERFAMILY SSF53732 Aconitase iron-sulfur domain
23 591 InterPro IPR036008 Aconitase, iron-sulfur domain
11 360 Gene3D G3DSA:3.30.499.10 Aconitase, domain 3
11 360 InterPro IPR015931 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
7 861 PANTHER PTHR11670 ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER
7 861 InterPro IPR006249 Aconitase/Iron-responsive element-binding protein 2
628 863 Gene3D G3DSA:3.20.19.10 Aconitase, domain 4
628 863 InterPro IPR015928 Aconitase/3-isopropylmalate dehydratase, swivel
361 565 Gene3D G3DSA:3.30.499.10 Aconitase, domain 3
361 565 InterPro IPR015931 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
469 482 PRINTS PR00415 Aconitase family signature
469 482 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
281 294 PRINTS PR00415 Aconitase family signature
281 294 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
205 220 PRINTS PR00415 Aconitase family signature
205 220 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
267 280 PRINTS PR00415 Aconitase family signature
267 280 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
191 204 PRINTS PR00415 Aconitase family signature
191 204 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
162 170 PRINTS PR00415 Aconitase family signature
162 170 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
407 418 PRINTS PR00415 Aconitase family signature
407 418 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
138 151 PRINTS PR00415 Aconitase family signature
138 151 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
360 374 PRINTS PR00415 Aconitase family signature
360 374 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
628 863 FunFam G3DSA:3.20.19.10:FF:000006 Aconitate hydratase 1
67 538 Pfam PF00330 Aconitase family (aconitate hydratase)
67 538 InterPro IPR001030 Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
10 360 FunFam G3DSA:3.30.499.10:FF:000015 Aconitate hydratase 1

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0794
AlphaFold full sequence Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.544

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

25 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ATH P20004 187.1 Da LogP -5.48 TPSA 140.6 ✓ Ro5 ✓ Clean C(=C(/[C@H](C(=O)[O-])O)\C(=O)[O-])/C(=O)[O-]
F3S P16276 295.8 Da LogP 2.59 TPSA 0.0 ✓ Ro5 ✓ Clean S1[Fe]2S[Fe]3[S]2[Fe]1S3
FLC P16276 189.1 Da LogP -5.25 TPSA 140.6 ✓ Ro5 ✓ Clean C(C(=O)[O-])C(CC(=O)[O-])(C(=O)[O-])O
ICT P16276 192.1 Da LogP -1.39 TPSA 132.1 ✓ Ro5 ✓ Clean C([C@@H]([C@H](C(=O)O)O)C(=O)O)C(=O)O
KP1 P81291 132.2 Da LogP 0.92 TPSA 40.5 ✓ Ro5 ✓ Clean CC(C)(CC(C)(C)O)O
MIC P20004 206.2 Da LogP -1.00 TPSA 132.1 ✓ Ro5 ✓ Clean C[C@@]([C@H](CC(=O)O)C(=O)O)(C(=O)O)O
NIC P20004 193.1 Da LogP -1.45 TPSA 138.0 ✓ Ro5 ✓ Clean C([C@@H]([C@H](C(=O)O)O)[N+](=O)[O-])C(=O)O
NTC P20004 193.1 Da LogP -1.45 TPSA 138.0 ✓ Ro5 ✓ Clean C(C(=O)O)[C@@](C[N+](=O)[O-])(C(=O)O)O
O P16276 18.0 Da LogP -0.82 TPSA 31.5 ✓ Ro5 ✓ Clean O
TRA P20004 171.1 Da LogP -4.45 TPSA 120.4 ✓ Ro5 ✓ Clean C(/C(=C\C(=O)[O-])/C(=O)[O-])C(=O)[O-]
TRC P16276 176.1 Da LogP -0.36 TPSA 111.9 ✓ Ro5 ✓ Clean C(C(CC(=O)O)C(=O)O)C(=O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.