Protein profile

PA0930

two-component sensor

Genome: NC_002516.2

Gene: PA0930 Structure source: AlphaFold UniProt Q9I528
Export view
Amino acids 445
Annotations 6
Features 45
PDB binders 4
Druggability 0.942

Overview

Basic information about this protein and its source genome.

Accession
PA0930
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA0930
Status
annotated
Amino acids
445
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor). GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.942
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

5
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

45 records
Show feature table
Start End DB Term Name
177 232 ProSiteProfiles PS50885 HAMP domain profile.
177 232 InterPro IPR003660 HAMP domain
7 29 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
39 148 Pfam PF16750 Sensor domain of 2-component histidine kinase
39 148 InterPro IPR031930 Two-component histidine kinase, sensor domain
340 431 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
340 431 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
177 232 SMART SM00304 HAMP_11
392 402 PRINTS PR00344 Bacterial sensor protein C-terminal signature
392 402 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
407 425 PRINTS PR00344 Bacterial sensor protein C-terminal signature
407 425 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
374 388 PRINTS PR00344 Bacterial sensor protein C-terminal signature
374 388 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
217 291 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
217 291 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
26 149 Gene3D G3DSA:3.30.450.170 -
26 149 InterPro IPR038428 Two-component histidine kinase, sensor domain superfamily
28 156 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
266 286 Coils Coil Coil
154 232 Gene3D G3DSA:6.10.340.10 -
174 228 Pfam PF00672 HAMP domain
174 228 InterPro IPR003660 HAMP domain
177 445 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
4 445 PANTHER PTHR45436 SENSOR HISTIDINE KINASE YKOH
240 445 ProSiteProfiles PS50109 Histidine kinase domain profile.
240 445 InterPro IPR005467 Histidine kinase domain
157 176 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
233 292 SMART SM00388 HisKA_10
233 292 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
1 6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
234 291 Pfam PF00512 His Kinase A (phospho-acceptor) domain
234 291 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
233 291 Gene3D G3DSA:1.10.287.130 -
177 229 SUPERFAMILY SSF158472 HAMP domain-like
7 27 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
182 227 CDD cd06225 HAMP
337 445 SMART SM00387 HKATPase_4
337 445 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
231 287 CDD cd00082 HisKA
231 287 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
282 443 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
282 443 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
321 445 Gene3D G3DSA:3.30.565.10 -
321 445 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA0930
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.636

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

54 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.