Protein profile

PA1249

alkaline metalloproteinase

Genome: NC_002516.2

Gene: aprA PA1249 Structure source: Experimental + AlphaFold UniProt Q03023
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Amino acids 479
Annotations 12
Features 27
PDB binders 0
Druggability 0.559

Overview

Basic information about this protein and its source genome.

Accession
PA1249
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
aprA PA1249
Status
annotated
Amino acids
479
Structure source
Experimental + AlphaFold
EC
3.4.24.40
GO
GO:0005615 OBSOLETE. That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid. GO:0005509 Binding to a calcium ion (Ca2+). GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid. GO:0008270 Binding to a zinc ion (Zn). GO:0045959 Any process that stops, prevents, or reduces the frequency, rate or extent of complement activation by the classical pathway.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Extracellular

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.559
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 11 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

11
  • GO:0005615 OBSOLETE. That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
  • GO:0005509 Binding to a calcium ion (Ca2+).
  • GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
  • GO:0008233 Catalysis of the hydrolysis of a peptide bond. A peptide bond is a covalent bond formed when the carbon atom from the carboxyl group of one amino acid shares electrons with the nitrogen atom from the amino group of a second amino acid.
  • GO:0008270 Binding to a zinc ion (Zn).
  • GO:0045959 Any process that stops, prevents, or reduces the frequency, rate or extent of complement activation by the classical pathway.
  • GO:0001869 Any process that stops, prevents, or reduces the rate of complement activation by the lectin pathway.
  • GO:0010765 Any process that increases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.
  • GO:0141141 A process by which a symbiont avoids recognition by host's innate immune response by altering or concealing a conserved molecule recognized by the host's cell surface and intracellular pattern recognition receptors, including Toll-like and NOD-like receptors. The host is defined as the larger of the organisms involved in a symbiotic interaction.
  • GO:0008237 Catalysis of the hydrolysis of peptide bonds by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.

Sequence Features

Domain/signature hits from InterPro and related databases.

27 records
Show feature table
Start End DB Term Name
27 259 Gene3D G3DSA:3.40.390.10 Collagenase (Catalytic Domain)
27 259 InterPro IPR024079 Metallopeptidase, catalytic domain superfamily
14 255 SUPERFAMILY SSF55486 Metalloproteases ("zincins"), catalytic domain
256 478 SUPERFAMILY SSF51120 beta-Roll
256 478 InterPro IPR011049 Serralysin-like metalloprotease, C-terminal
21 476 Gene3D G3DSA:2.150.10.10 -
21 476 InterPro IPR011049 Serralysin-like metalloprotease, C-terminal
257 479 Pfam PF08548 Peptidase M10 serralysin C terminal
257 479 InterPro IPR013858 Peptidase M10 serralysin, C-terminal
14 479 NCBIfam NF035945 serralysin family metalloprotease
27 259 FunFam G3DSA:3.40.390.10:FF:000046 Serralysin
170 230 Pfam PF13583 Metallo-peptidase family M12B Reprolysin-like
65 256 CDD cd04277 ZnMc_serralysin_like
65 256 InterPro IPR034033 Serralysin-like metallopeptidase domain
369 377 PRINTS PR00313 NodO calcium binding signature
339 350 PRINTS PR00313 NodO calcium binding signature
360 368 PRINTS PR00313 NodO calcium binding signature
378 387 PRINTS PR00313 NodO calcium binding signature
254 476 FunFam G3DSA:2.150.10.10:FF:000001 Serralysin
365 383 ProSitePatterns PS00330 Hemolysin-type calcium-binding region signature.
365 383 InterPro IPR018511 Hemolysin-type calcium-binding conserved site
78 241 SMART SM00235 col_5
78 241 InterPro IPR006026 Peptidase, metallopeptidase
350 384 Pfam PF00353 RTX calcium-binding nonapeptide repeat (4 copies)
350 384 InterPro IPR001343 RTX calcium-binding nonapeptide repeat
1 479 PIRSF PIRSF001205 Serralysin
1 479 InterPro IPR016294 Peptidase M10B

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

4 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 1KAP
X-ray 1.64 Å P
100.0% 1-479
PDBe RCSB PDBj File
Viewing
PDB 1JIW
X-ray 1.74 Å P
98.1% 10-479
PDBe RCSB PDBj File
Loaded
PDB 1AKL
X-ray 2.00 Å A
98.1% 10-479
PDBe RCSB PDBj File
Loaded
PDB 3VI1
X-ray 2.00 Å A,B
98.1% 10-479
PDBe RCSB PDBj File
Loaded
AlphaFold PA1249
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.559
1 0.429

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 32.37 0.943
2 1.99 0.042
3 1.85 0.036
4 1.54 0.023
5 1.19 0.01