Protein profile

PA1250

alkaline proteinase inhibitor AprI

Genome: NC_002516.2

Gene: aprI PA1250 inh Structure source: Experimental + AlphaFold UniProt Q03026
Export view
Amino acids 131
Annotations 4
Features 20
PDB binders 0
Druggability 0.411

Overview

Basic information about this protein and its source genome.

Accession
PA1250
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
aprI PA1250 inh
Status
annotated
Amino acids
131
Structure source
Experimental + AlphaFold
GO
GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria). GO:0008191 Binds to and stops, prevents or reduces the activity of metalloendopeptidases, enzymes that catalyze the hydrolysis of nonterminal peptide bonds in a polypeptide chain and contain a chelated metal ion at their active sites which is essential to their catalytic activity. GO:0004866 Binds to and stops, prevents or reduces the activity of an endopeptidase. GO:0010951 Any process that decreases the frequency, rate or extent of endopeptidase activity, the endohydrolysis of peptide bonds within proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Periplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.411
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

4 GO

Gene Ontology (GO)

4
  • GO:0042597 The region between the inner (cytoplasmic) and outer membrane (Gram-negative Bacteria) or cytoplasmic membrane and cell wall (Fungi and Gram-positive Bacteria).
  • GO:0008191 Binds to and stops, prevents or reduces the activity of metalloendopeptidases, enzymes that catalyze the hydrolysis of nonterminal peptide bonds in a polypeptide chain and contain a chelated metal ion at their active sites which is essential to their catalytic activity.
  • GO:0004866 Binds to and stops, prevents or reduces the activity of an endopeptidase.
  • GO:0010951 Any process that decreases the frequency, rate or extent of endopeptidase activity, the endohydrolysis of peptide bonds within proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
1 27 Phobius SIGNAL_PEPTIDE Signal peptide region
1 25 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM
26 42 PRINTS PR01274 Metalloprotease inhibitor signature
26 42 InterPro IPR022815 Protease inhibitor
68 79 PRINTS PR01274 Metalloprotease inhibitor signature
68 79 InterPro IPR022815 Protease inhibitor
90 103 PRINTS PR01274 Metalloprotease inhibitor signature
90 103 InterPro IPR022815 Protease inhibitor
107 120 PRINTS PR01274 Metalloprotease inhibitor signature
107 120 InterPro IPR022815 Protease inhibitor
26 128 SUPERFAMILY SSF50882 beta-Barrel protease inhibitors
26 128 InterPro IPR016085 Protease inhibitor, beta-barrel domain
11 22 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 10 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
32 128 Pfam PF02974 Protease inhibitor Inh
32 128 InterPro IPR021140 Alkaline proteinase inhibitor/ Outer membrane lipoprotein Omp19
23 27 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
12 34 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
28 131 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
26 131 Gene3D G3DSA:2.40.128.10 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 1JIW
X-ray 1.74 Å I
80.9% 26-131
PDBe RCSB PDBj File
Viewing
AlphaFold PA1250
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer