Protein target profile

PA1270

hypothetical protein

Genome: NC_002516.2

Gene: PA1270 3D evidence: AlphaFold DB model UniProt Q9I474
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Length 679
Pocket druggability 0.694
EC / GO 0 / 3
Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

3 signals

Strengths

No similar human protein detected The current BLAST sequence screen did not find a significant human match, lowering apparent off-target risk. Moderate predicted binding pocket FPocket selected-pocket score 0.69. This supports review but is not experimental validation. Structure available AlphaFold DB model

Risks / watch

No major risk signal surfaced in the loaded evidence.

Missing evidence

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein. No ligand evidence No experimental PDB ligands, measured ChEMBL bioactivity, or proposed ZINC compound records are available.
How to read this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal TPW pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Overview

Basic information about this protein and its source genome.

Accession
PA1270
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA1270
Status
annotated
Amino acids
679
3D evidence
AlphaFold DB model
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
Hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected pocket evidence

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.694
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MAKPSFFSSDLVQVLLCPRLHELQFAVKALLAGGLALYLAFGLELEQPQWALMTVFVVSQPYSGMVLAKGMFRLIGTCAGALVSIGMVALYGQASLPFLLLMALWLAFCTAGASLLHNHASYGFVLAGYTAAIVALPASADPATVFDQAVARCSEIGLGILCAALVNVLLWPRRLERQLANQGKAAWEAGLQAAAAELRGADERGELLAALGRIAAADAQRDHAWFEGALGRARSQALRVLGLDLLGLLRAARKVARERRLLDPASAHALQPWLEELEALLREGRQAEFEACRQRLRLALDAAHGQLHLCLVCLERLLGEARAAGRSVEALVYGAPTRQAPGAIAWHRDIERGVLFGLRSALAFLCVAAFWLASAWPSGLGAVSITGVVLSLFASRDNPAQAGLNFLRGILLSIPLAGFVALFYLPGVDGFPLLCLGLGVPLFFAALCVNRASLAGIASPFCIFFVKNVAPSNSMSYDLAHFLNNALSTVLGVAFAVLVFNLVSLRPGERHYRRMLQATLGDLARLTLRSPAQAEAWFGGRTADRLIRLAQRYDRLPEGRRQPWSDGLMGLDFGDELLYLRQCLEEVPASLAQARDRYLRRLRLALLGDGPRAEREHALDPPTARLLKALAASPLAGSERGELAGAALVQLQATWRQWCRSHAPAGTALRADPLPGAGR

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

3 GO

Gene Ontology (GO)

3
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

38 records
Show feature table
Start End DB Term Name
98 116 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
426 430 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
74 92 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
149 171 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
23 45 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
117 122 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
431 449 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 24 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
443 465 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
96 115 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
93 97 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
144 148 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
123 143 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
122 139 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
506 679 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
354 372 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
454 470 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
23 554 PANTHER PTHR30509 P-HYDROXYBENZOIC ACID EFFLUX PUMP SUBUNIT-RELATED
23 660 Pfam PF04632 Fusaric acid resistance protein family
23 660 InterPro IPR006726 Para-hydroxybenzoic acid efflux pump subunit AaeB/fusaric acid resistance protein
471 481 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
373 377 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
44 48 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
395 405 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
68 73 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
49 67 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
149 171 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
486 505 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
25 43 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
482 505 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
70 92 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
376 393 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
378 394 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
450 453 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
355 372 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
172 353 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
406 425 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
406 428 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

3D visualization script Full viewer

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Structural evidence

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Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold DB PA1270
AlphaFold DB — — full sequence — Viewing
Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #7
0.694
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Site 2 FPocket #5
0.628
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Site 3 FPocket #27
0.42
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Site 4 FPocket #2
0.288
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