Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA1273
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: cobB PA1273
Status: annotated
Amino acids: 435
Structure source: AlphaFold

EC

6.3.5.9

GO

GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0042242 Catalysis of the conversion of cobyrinic acid to cobyrinic acid a,c-diamide via the intermediate formation of cobyrinic acid c-monoamide. GO:0043802 Catalysis of the reaction: 2 L-glutamine + 2 ATP + 2 H2O + hydrogenobyrinate = 2 L-glutamate + 2 ADP + 4 H+ + hydrogenobyrinate a,c-diamide + 2 phosphate. GO:0009236 The chemical reactions and pathways resulting in the formation of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): N
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.682

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

MTQARCPALLIAAPASGQGKTTVTAALARLHARQGRRVRVFKCGPDFLDPMILARASGAPVYQLDLWMVGEAEARRLLARAAGEADLILIEGVMGLFDGNPSAADLARRFGVPVLGVINGAAMAQTFGALAYGLAHFQPDLPFSGVLGNRVGSQRHSDILRDCLPPGMRWFGGLPRSAEFELPSRHLGLVQAEELADLDARLDAAADALRASAETDLPEPVTFEVPAPAPLQRSLEGVRIGVARDASFAFLYQANLDLLRELGAELAFFSPLQDQALPAVDSLYLPGGYPELHLGRLQGNRAMAEAIRAHHAAGKPLLAECGGMLYLLDCLEDADGERGELLGLLPGRARLQKRLTALALQEVELPEGRLRGHTFHHSTLDCAVEPLARGVCPNGRNTAEAVFRLGRLTASYIHFYLPSNPQAAAALLAPARDAD

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 5 GO

Enzyme Commission (EC)

1

6.3.5.9

Gene Ontology (GO)

5

GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0042242 Catalysis of the conversion of cobyrinic acid to cobyrinic acid a,c-diamide via the intermediate formation of cobyrinic acid c-monoamide.
GO:0043802 Catalysis of the reaction: 2 L-glutamine + 2 ATP + 2 H2O + hydrogenobyrinate = 2 L-glutamate + 2 ADP + 4 H+ + hydrogenobyrinate a,c-diamide + 2 phosphate.
GO:0009236 The chemical reactions and pathways resulting in the formation of cobalamin (vitamin B12), a water-soluble vitamin characterized by possession of a corrin nucleus containing a cobalt atom.
GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Sequence Features

Domain/signature hits from InterPro and related databases.

26 records

Show feature table

Start	End	DB	Term	Name
236	414	Gene3D	G3DSA:3.40.50.880	-
236	414	InterPro	IPR029062	Class I glutamine amidotransferase-like
9	187	Pfam	PF01656	CobQ/CobB/MinD/ParA nucleotide binding domain
9	187	InterPro	IPR002586	CobQ/CobB/MinD/ParA nucleotide binding domain
239	422	ProSiteProfiles	PS51274	CobBQ-type GATase domain profile.
14	17	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
1	17	Phobius	SIGNAL_PEPTIDE	Signal peptide region
1	5	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
7	432	PANTHER	PTHR43873	COBYRINATE A,C-DIAMIDE SYNTHASE
7	432	InterPro	IPR004484	Cobyrinic acid a,c-diamide synthase CbiA
6	421	Hamap	MF_00027	Hydrogenobyrinate a,c-diamide synthase [cobB].
6	421	InterPro	IPR004484	Cobyrinic acid a,c-diamide synthase CbiA
10	430	NCBIfam	TIGR00379	hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing)
10	430	InterPro	IPR004484	Cobyrinic acid a,c-diamide synthase CbiA
7	191	CDD	cd05388	CobB_N
239	418	Pfam	PF07685	CobB/CobQ-like glutamine amidotransferase domain
239	418	InterPro	IPR011698	CobB/CobQ-like glutamine amidotransferase
45	182	Gene3D	G3DSA:3.40.50.300	-
45	182	InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
235	428	SUPERFAMILY	SSF52317	Class I glutamine amidotransferase-like
235	428	InterPro	IPR029062	Class I glutamine amidotransferase-like
240	421	CDD	cd03130	GATase1_CobB
8	194	SUPERFAMILY	SSF52540	P-loop containing nucleoside triphosphate hydrolases
8	194	InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
6	13	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.
18	435	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA1273	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.682
3				0.438
2				0.371

PA1273