Protein profile

PA1396

two-component sensor

Genome: NC_002516.2

Gene: PA1396 Structure source: AlphaFold UniProt Q9I3U8
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Amino acids 540
Annotations 10
Features 54
PDB binders 3
Druggability 0.738

Overview

Basic information about this protein and its source genome.

Accession
PA1396
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA1396
Status
annotated
Amino acids
540
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0030295 Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein. GO:0007234 The series of molecular signals generated in response to osmotic change, as mediated through a phosphorelay system. In S.cerevisiae, the osmosensor is the histidine kinase Sln1. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.738
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

MKFEKNTELDQANLRLIVATCAILYVVLIGLLPGLKVETYLPIVAYYGLFLIASILLRQAIVRWPGHYPARRIFCMLHDYAGTSFGLIVGGEAALPLYAVMVWINLGNGMRYGSRYLAIATALALLALLVIYRLTPAWQAQPFMVLMLMTTSTVIPFYAHLLLERTRKATEEALQANQEKSRLLAQASHDLRQPIHSIGLFTACLRDARLGDEERRLVDNIDRSLLNVSQLFRSILDLYTLDNGRLQPKQENVHLGELLRDLVRRNAEAARWAGVELRLRPCRLWTRTDPGLLSTMLQNLLSNSFKYAAERPLLIGVRRRGDGLAVAIYDQGRGIAEEHLPRVFEEFYRVRETRDRDVEGIGLGLSIVRRLGQLTGIEVTLRSRVGRGTAVTLHGLPAVAAQALPRRDDPLQAGLLTGLRVCLVEDDRNVLRATSALLERWGCTVQAETEADGWRTDCDILVVDYDLGPHASGVECIERVRRQRGEAIPALVISGHDIERIQASVEDTDIALLSKPVRPTELRATLRALRERPVTASHAS

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

9
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0030295 Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.
  • GO:0007234 The series of molecular signals generated in response to osmotic change, as mediated through a phosphorelay system. In S.cerevisiae, the osmosensor is the histidine kinase Sln1.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

54 records
Show feature table
Start End DB Term Name
140 159 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
419 526 SMART SM00448 REC_2
419 526 InterPro IPR001789 Signal transduction response regulator, receiver domain
289 394 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
289 394 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
12 32 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
249 398 Gene3D G3DSA:3.30.565.10 -
249 398 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
116 134 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
168 393 PANTHER PTHR42878 TWO-COMPONENT HISTIDINE KINASE
114 136 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
82 104 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
180 244 Pfam PF00512 His Kinase A (phospho-acceptor) domain
180 244 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
423 516 CDD cd00156 REC
135 139 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
359 377 PRINTS PR00344 Bacterial sensor protein C-terminal signature
359 377 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
342 352 PRINTS PR00344 Bacterial sensor protein C-terminal signature
342 352 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
324 338 PRINTS PR00344 Bacterial sensor protein C-terminal signature
324 338 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
416 531 Gene3D G3DSA:3.40.50.2300 -
1 11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
179 244 SMART SM00388 HisKA_10
179 244 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
44 61 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
33 43 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
143 162 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
105 115 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
186 393 ProSiteProfiles PS50109 Histidine kinase domain profile.
186 393 InterPro IPR005467 Histidine kinase domain
81 104 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
249 398 FunFam G3DSA:3.30.565.10:FF:000460 Probable two-component sensor
160 242 Gene3D G3DSA:1.10.287.130 -
177 238 CDD cd00082 HisKA
177 238 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
288 399 SMART SM00387 HKATPase_4
288 399 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
421 526 Pfam PF00072 Response regulator receiver domain
421 526 InterPro IPR001789 Signal transduction response regulator, receiver domain
163 245 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
163 245 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
160 540 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
166 186 Coils Coil Coil
62 80 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
234 394 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
234 394 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
420 530 ProSiteProfiles PS50110 Response regulatory domain profile.
420 530 InterPro IPR001789 Signal transduction response regulator, receiver domain
417 532 SUPERFAMILY SSF52172 CheY-like
417 532 InterPro IPR011006 CheY-like superfamily
40 62 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA1396
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.218

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
BEF
5N2N
O22267 66.0 Da LogP 0.88 TPSA 0.0 ✓ Ro5 ✓ Clean [Be-](F)(F)F
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.