Protein target profile

PA1528

cell division protein ZipA

Genome: NC_002516.2

Gene: PA1528 zipA 3D evidence: AlphaFold DB model UniProt Q9I3I5

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Length 289

Pocket druggability 0.696

Ligand records 11

EC / GO 0 / 6

Target summary

Target candidate with partial support; inspect missing evidence before prioritizing.

4 signals

Strengths

Risks / watch

No major risk signal surfaced in the loaded evidence.

Missing evidence

No metabolic context No SBML/network-derived reaction evidence is loaded for this protein.

How to read this page

PDB: experimentally determined structures from the Protein Data Bank. These are the strongest structural evidence, but may cover only part of the protein.

AlphaFold DB model: a precomputed predicted structure downloaded from AlphaFold Database/UniProt, not an experiment performed here.

ColabFold model: a predicted structure generated for this workspace; interpret it with coverage and confidence.

pLDDT: confidence score for predicted structures. High values support local geometry; low values mean the region should not drive pocket interpretation.

FPocket / P2Rank: software tools that predict possible ligand-binding pockets on a 3D structure. They are useful screening signals, not experimental validation.

Druggability: a pocket-based estimate of whether a small molecule could bind productively. It does not mean a drug already exists.

PDB ligand: a compound observed in an experimental structure. Direct same-protein records are stronger than homolog-transferred records.

ChEMBL: a public database of measured compound bioactivity. Direct entries are stronger than entries transferred from similar proteins.

ZINC: a purchasable-compound database. Here it marks proposed candidates from chemical similarity, not measured binders.

LigQ / LigQ_2: an internal TPW pipeline step that gathers PDB, ChEMBL, and ZINC ligand evidence for each protein.

Off-target: sequence similarity to proteins we prefer not to hit, such as human proteins or beneficial gut microbiome proteins.

DEG: Database of Essential Genes. A match suggests the protein resembles genes known to be essential in other organisms.

Roary / CoreCruncher: pan-genome tools used to decide whether a gene is core across analyzed strains or accessory/strain-specific.

EC / GO: functional annotations: EC describes enzyme reactions; GO describes biological process, molecular function, or cellular component.

KEGG pathway: a curated metabolic route label used here to group reactions imported from the metabolic model.

Chokepoint: a metabolic reaction that is the only producer or consumer of a metabolite in the imported model.

Overview

Basic information about this protein and its source genome.

Accession: PA1528
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA1528 zipA
Status: annotated
Amino acids: 289
3D evidence: AlphaFold DB model

GO:0032153 The eventual plane of cell division (also known as cell cleavage or cytokinesis) in a dividing cell. In Eukaryotes, the cleavage apparatus, composed of septin structures and the actomyosin contractile ring, forms along this plane, and the mitotic, or meiotic, spindle is aligned perpendicular to the division plane. In bacteria, the cell division site is generally located at mid-cell and is the site at which the cytoskeletal structure, the Z-ring, assembles. GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0000917 The assembly and arrangement of a septum that spans the plasma membrane interface between progeny cells following cytokinesis. The progeny cells that form a division septum are not able to exchange intracellular material. GO:0043093 A cytokinesis process that involves a set of conserved proteins including FtsZ, and results in the formation of two similarly sized and shaped cells. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0090529 The assembly and arrangement of a cellular component that is composed of peptidoglycan and often chitin in addition to other materials and usually forms perpendicular to the long axis of a cell or hypha. It grows centripetally from the cell wall to the center of the cell and often functions in the compartmentalization of a cell into two daughter cells.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: Hit
Essential (DEG): Y
Localization: CytoplasmicMembrane

Selected pocket evidence

The selected pocket score is the FPocket value used for ranking after applying the curated structure priority. It estimates small-molecule pocket quality; it is not experimental binding evidence. The 3D viewer may show a different loaded structure, so visible pockets can differ.

FPocket 0.696

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

MDIGLREWLIVIGLIVIAGILFDGWRRMRGGKGKLKFKLDRSFANLPDDDGDSAELLGPARVVEHREPSFDEQDLPSVSAREAKERKGGKRQEEPRQGDLDLDEGLALEADPSDAAETVEPRKGKSKGRKEKEREKAPAVAAEPAPVDEVLIINVIARDESGFKGPALLQNILESGLRFGDMDIFHRHESMAGNGEILFSMANAVKPGTFDLDDIDNFSTRAVSFFLGLPGPRHPKQAFDVMVAAARKLAHELNGELKDEQRSVLTAQTIEHYRQRIIDHERRSLMQKR

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

6 GO

Gene Ontology (GO)

GO:0032153 The eventual plane of cell division (also known as cell cleavage or cytokinesis) in a dividing cell. In Eukaryotes, the cleavage apparatus, composed of septin structures and the actomyosin contractile ring, forms along this plane, and the mitotic, or meiotic, spindle is aligned perpendicular to the division plane. In bacteria, the cell division site is generally located at mid-cell and is the site at which the cytoskeletal structure, the Z-ring, assembles.
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO:0000917 The assembly and arrangement of a septum that spans the plasma membrane interface between progeny cells following cytokinesis. The progeny cells that form a division septum are not able to exchange intracellular material.
GO:0043093 A cytokinesis process that involves a set of conserved proteins including FtsZ, and results in the formation of two similarly sized and shaped cells.
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
GO:0090529 The assembly and arrangement of a cellular component that is composed of peptidoglycan and often chitin in addition to other materials and usually forms perpendicular to the long axis of a cell or hypha. It grows centripetally from the cell wall to the center of the cell and often functions in the compartmentalization of a cell into two daughter cells.

Sequence Features

Domain/signature hits from InterPro and related databases.

25 records

Show feature table

Start	End	DB	Term	Name
147	283	SUPERFAMILY	SSF64383	Cell-division protein ZipA, C-terminal domain
147	283	InterPro	IPR036765	ZipA, C-terminal FtsZ-binding domain superfamily
149	276	Pfam	PF04354	ZipA, C-terminal FtsZ-binding domain
149	276	InterPro	IPR007449	ZipA, C-terminal FtsZ-binding domain
6	25	Phobius	TRANSMEMBRANE	Region of a membrane-bound protein predicted to be embedded in the membrane.
5	277	NCBIfam	TIGR02205	cell division protein ZipA
5	277	InterPro	IPR011919	Cell division protein ZipA
1	5	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
26	289	Phobius	CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
7	25	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
243	263	Coils	Coil	Coil
115	139	MobiDBLite	mobidb-lite	consensus disorder prediction
148	277	CDD	cd00231	ZipA
148	277	InterPro	IPR007449	ZipA, C-terminal FtsZ-binding domain
147	284	Gene3D	G3DSA:3.30.1400.10	-
147	284	InterPro	IPR036765	ZipA, C-terminal FtsZ-binding domain superfamily
1	286	PANTHER	PTHR38685	CELL DIVISION PROTEIN ZIPA
1	286	InterPro	IPR011919	Cell division protein ZipA
55	141	MobiDBLite	mobidb-lite	consensus disorder prediction
147	277	SMART	SM00771	ZipA_C_cls
147	277	InterPro	IPR007449	ZipA, C-terminal FtsZ-binding domain
3	279	Hamap	MF_00509	Cell division protein ZipA [zipA].
3	279	InterPro	IPR011919	Cell division protein ZipA
59	103	MobiDBLite	mobidb-lite	consensus disorder prediction
147	284	FunFam	G3DSA:3.30.1400.10:FF:000002	Cell division protein ZipA

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; AlphaFold DB and ColabFold models typically cover the full protein but remain computational predictions.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries plus predicted AlphaFold DB or ColabFold models. Click Switch to display a different loaded structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold DB PA1528	AlphaFold DB	—	—	full sequence	—	Viewing

Pocket details Inspect a specific pocket, or open the full viewer

Binding pockets · FPocket

Druggability: high ≥ 0.7 · medium 0.4–0.69 · low < 0.4

Site 1 FPocket #1

0.696

Show in viewer

Site 2 FPocket #5

0.315

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Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

11 records

Chemistry signal

Structural ligand evidence is available for this target.

Direct evidence 0 11 via homologs

Structural ligands 4 0 loaded crystals

Bioactive compounds 0 7 ZINC proposed compounds

Drug-like & clean 7 4 PAINS alerts

Best available ligand signal

CL3 PDB via homolog 343.4 Da · LogP 3.44 · TPSA 63.4 Open detail RCSB PDB

Detail RCSB PDB CL3 PDB via homolog

Detail RCSB PDB IQZ PDB via homolog

Detail RCSB PDB WAC PDB via homolog

Detail RCSB PDB WAI PDB via homolog

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

No PDB structure with a co-crystallized ligand found for this exact protein.

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Ligand	Source crystal	UniProt (homolog)	MW · LogP · TPSA	Lipinski	PAINS	SMILES
CL3 RCSB PDB	1Y2G	P77173	343.4 Da LogP 3.44 TPSA 63.4	✓ Ro5	✓ Clean	`CC(=O)N(C)c1cccc(c1)c2nnc3n2nc(cc3)c4ccccc4`
IQZ RCSB PDB	1S1J	P77173	240.3 Da LogP 2.89 TPSA 36.1	✓ Ro5	✓ Clean	`c1ccc2c(c1)c3c([nH]2)[C@@H]4CCCC[N@]4CC3=O`
WAC RCSB PDB	1S1S	P77173	403.5 Da LogP 3.08 TPSA 71.4	✓ Ro5	✓ Clean	`CC(C)S(=O)(=O)NCCCn1c2ccccc2c3c1[C@@H]4CCCC[N@]…`
WAI RCSB PDB	1Y2F	P77173	424.0 Da LogP 3.32 TPSA 83.2	✓ Ro5	✓ Clean	`Cc1ccc(cc1Cl)Nc2nccc(n2)c3ccnc(c3)N4CCN(CC4)CCN`

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

Ligand	Tanimoto	MW · LogP · TPSA	Lipinski	PAINS	SMILES
ZINC105343 ZINC	0.755	281.3 Da LogP 2.08 TPSA 63.4	✓ Ro5	✓ Clean	`CC(=O)N(C)c1cccc(-c2ccc3nnc(C)n3n2)c1`
ZINC1630521 ZINC	0.533	212.3 Da LogP 2.86 TPSA 19.0	✓ Ro5	Alert	`c1ccc2c3c([nH]c2c1)[C@@H]1CCCN1CC3`
ZINC6576349 ZINC	0.533	212.3 Da LogP 2.86 TPSA 19.0	✓ Ro5	Alert	`c1ccc2c3c([nH]c2c1)[C@H]1CCCN1CC3`
ZINC1417172 ZINC	0.509	302.3 Da LogP 3.47 TPSA 52.3	✓ Ro5	✓ Clean	`COc1ccc(-c2nnc3ccc(-c4ccccc4)nn23)cc1`
ZINC1670185 ZINC	0.500	226.3 Da LogP 3.08 TPSA 19.0	✓ Ro5	Alert	`c1ccc2c3c([nH]c2c1)CN1CCCC[C@H]1C3`
ZINC20095944 ZINC	0.500	210.2 Da LogP 2.10 TPSA 43.1	✓ Ro5	✓ Clean	`Cc1nnc2ccc(-c3ccccc3)nn12`
ZINC4783271 ZINC	0.500	226.3 Da LogP 3.08 TPSA 19.0	✓ Ro5	Alert	`c1ccc2c3c([nH]c2c1)CN1CCCC[C@@H]1C3`

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.