Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA1546
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: hemN PA1546
Status: annotated
Amino acids: 460
Structure source: AlphaFold

EC

1.3.98.3

GO

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands. GO:0051989 Catalysis of the reaction: coproporphyrinogen III + 2 S-adenosyl-L-methionine = protoporphyrinogen IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine. GO:0004109 Catalysis of the reaction: coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX. GO:0046872 Binding to a metal ion. GO:0006779 The chemical reactions and pathways resulting in the formation of any member of a large group of derivatives or analogs of porphyrin. Porphyrin consists of a ring of four pyrrole nuclei linked each to the next at their alpha positions through a methine group.

Target profile

Computed evidence for target prioritization.

Human off-target: hit
Human identity (%): 30.645
Human E-value: 4.3e-20
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.823

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 9 GO

Enzyme Commission (EC)

1

1.3.98.3

Gene Ontology (GO)

9

GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0051539 Binding to a 4 iron, 4 sulfur (4Fe-4S) cluster; this cluster consists of four iron atoms, with the inorganic sulfur atoms found between the irons and acting as bridging ligands.
GO:0051989 Catalysis of the reaction: coproporphyrinogen III + 2 S-adenosyl-L-methionine = protoporphyrinogen IX + 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine.
GO:0004109 Catalysis of the reaction: coproporphyrinogen III + 2 H+ + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX.
GO:0046872 Binding to a metal ion.
GO:0006779 The chemical reactions and pathways resulting in the formation of any member of a large group of derivatives or analogs of porphyrin. Porphyrin consists of a ring of four pyrrole nuclei linked each to the next at their alpha positions through a methine group.
GO:0006782 The chemical reactions and pathways resulting in the formation of protoporphyrinogen IX.
GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
GO:0051536 Binding to an iron-sulfur cluster, a combination of iron and sulfur atoms.

Sequence Features

Domain/signature hits from InterPro and related databases.

24 records

Show feature table

Start	End	DB	Term	Name
63	255	CDD	cd01335	Radical_SAM
14	448	PANTHER	PTHR13932	COPROPORPHYRINIGEN III OXIDASE
14	448	InterPro	IPR034505	Anaerobic coproporphyrinogen-III oxidase
53	276	SMART	SM00729	MiaB
53	276	InterPro	IPR006638	Elp3/MiaA/NifB-like, radical SAM core domain
61	284	Gene3D	G3DSA:3.80.30.20	tm_1862 like domain
61	284	InterPro	IPR023404	Radical SAM, alpha/beta horseshoe
3	460	SFLD	SFLDG01065	anaerobic coproporphyrinogen-III oxidase like
63	254	SFLD	SFLDG01082	B12-binding domain containing
368	460	FunFam	G3DSA:1.10.10.920:FF:000002	Coproporphyrinogen-III oxidase
5	448	SUPERFAMILY	SSF102114	Radical SAM enzymes
3	459	PIRSF	PIRSF000167	HemN
3	459	InterPro	IPR004558	Oxygen-independent coproporphyrinogen III oxidase HemN
6	460	NCBIfam	TIGR00538	oxygen-independent coproporphyrinogen III oxidase
6	460	InterPro	IPR004558	Oxygen-independent coproporphyrinogen III oxidase HemN
3	460	SFLD	SFLDF00277	oxygen-independent coproporphyrinogen-III oxidase 1 (HemN-like)
3	460	InterPro	IPR004558	Oxygen-independent coproporphyrinogen III oxidase HemN
58	232	Pfam	PF04055	Radical SAM superfamily
58	232	InterPro	IPR007197	Radical SAM
368	460	Gene3D	G3DSA:1.10.10.920	-
48	287	ProSiteProfiles	PS51918	Radical SAM core domain profile.
48	287	InterPro	IPR007197	Radical SAM
369	435	Pfam	PF06969	HemN C-terminal domain
369	435	InterPro	IPR010723	HemN, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA1546	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.616
2				0.502

PA1546