Protein profile

PA1783

nitrate transporter

Genome: NC_002516.2

Gene: nasA PA1783 Structure source: AlphaFold UniProt Q9I2V9
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Amino acids 403
Annotations 9
Features 51
PDB binders 2
Druggability 0.79

Overview

Basic information about this protein and its source genome.

Accession
PA1783
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
nasA PA1783
Status
annotated
Amino acids
403
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0015112 Enables the transfer of nitrate ions (NO3-) from one side of a membrane to the other. GO:0015113 Enables the transfer of nitrite (NO2-) ions from one side of a membrane to the other. GO:0042128 The nitrogen metabolic process that encompasses the uptake of nitrate from the environment and reduction to ammonia, and results in the incorporation of nitrogen derived from nitrate into cellular substances. GO:0015706 The directed movement of nitrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.79
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0015112 Enables the transfer of nitrate ions (NO3-) from one side of a membrane to the other.
  • GO:0015113 Enables the transfer of nitrite (NO2-) ions from one side of a membrane to the other.
  • GO:0042128 The nitrogen metabolic process that encompasses the uptake of nitrate from the environment and reduction to ammonia, and results in the incorporation of nitrogen derived from nitrate into cellular substances.
  • GO:0015706 The directed movement of nitrate into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
  • GO:0015707 The directed movement of nitrite into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Sequence Features

Domain/signature hits from InterPro and related databases.

51 records
Show feature table
Start End DB Term Name
266 276 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
247 265 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
50 71 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
72 77 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
212 235 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
32 50 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
14 379 CDD cd17341 MFS_NRT2_like
14 379 InterPro IPR044772 Nitrate transporter
51 71 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
98 102 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
366 385 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
127 137 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
78 97 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
12 31 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
103 126 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
236 246 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
13 347 NCBIfam TIGR00886 NarK family nitrate/nitrite MFS transporter
13 347 InterPro IPR004737 Nitrate transporter NarK/NarU-like
2 390 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains
2 390 InterPro IPR036259 MFS transporter superfamily
192 211 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
169 191 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
297 301 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
138 163 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
168 190 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
272 294 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
78 97 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
164 168 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
277 296 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
23 348 Pfam PF07690 Major Facilitator Superfamily
23 348 InterPro IPR011701 Major facilitator superfamily
386 403 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
299 321 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
9 374 PANTHER PTHR23515 HIGH-AFFINITY NITRATE TRANSPORTER 2.3
9 374 InterPro IPR044772 Nitrate transporter
332 354 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
142 164 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
6 390 SUPERFAMILY SSF103473 MFS general substrate transporter
6 390 InterPro IPR036259 MFS transporter superfamily
13 35 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
302 320 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
107 129 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
366 385 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
334 356 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
355 365 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
243 265 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
13 390 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile.
13 390 InterPro IPR020846 Major facilitator superfamily domain
321 331 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
211 233 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA1783
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.79
2 0.467

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
OLA
4U4T
P10903 282.5 Da LogP 6.11 TPSA 37.3 1 viol. ✓ Clean CCCCCCCC\C=C/CCCCCCCC(=O)O
OLC
4U4T
P10903 356.5 Da LogP 4.92 TPSA 66.8 ✓ Ro5 ✓ Clean CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](CO)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.