Protein profile

PA1871

protease LasA

Genome: NC_002516.2

Gene: lasA PA1871 Structure source: Experimental + AlphaFold UniProt P14789
Export view
Amino acids 418
Annotations 9
Features 26
PDB binders 1
Druggability 0.52

Overview

Basic information about this protein and its source genome.

Accession
PA1871
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
lasA PA1871
Status
annotated
Amino acids
418
Structure source
Experimental + AlphaFold
EC
3.4.24.-
GO
GO:0005615 OBSOLETE. That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid. GO:0004175 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain. GO:0046872 Binding to a metal ion. GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions. GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides. GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
OuterMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.52
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005615 OBSOLETE. That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid.
  • GO:0004175 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain.
  • GO:0046872 Binding to a metal ion.
  • GO:0004222 Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, one or two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions.
  • GO:0009253 The chemical reactions and pathways resulting in the breakdown of peptidoglycans, any of a class of glycoconjugates found in bacterial cell walls and consisting of long glycan strands of alternating residues of beta-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid, cross-linked by short peptides.
  • GO:0015628 The process in which proteins are secreted across the outer membrane of Gram-negative bacteria by the type II secretion system. Proteins using this pathway are first translocated across the cytoplasmic membrane via the Sec or Tat pathways.
  • GO:0043952 The process in which unfolded proteins are transported across the cytoplasmic membrane in Gram-positive and Gram-negative bacteria by the Sec complex, in a process involving proteolytic cleavage of an N-terminal signal peptide.
  • GO:0006508 The hydrolysis of proteins into smaller polypeptides and/or amino acids by cleavage of their peptide bonds.

Sequence Features

Domain/signature hits from InterPro and related databases.

26 records
Show feature table
Start End DB Term Name
16 26 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
1 15 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
237 418 FunFam G3DSA:2.70.70.10:FF:000044 Protease LasA
1 31 Phobius SIGNAL_PEPTIDE Signal peptide region
237 418 Gene3D G3DSA:2.70.70.10 Glucose Permease (Domain IIA)
237 418 InterPro IPR011055 Duplicated hybrid motif
287 361 CDD cd12797 M23_peptidase
286 367 Pfam PF01551 Peptidase family M23
286 367 InterPro IPR016047 Peptidase M23
1 31 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM
205 368 PANTHER PTHR21666 PEPTIDASE-RELATED
226 365 SUPERFAMILY SSF51261 Duplicated hybrid motif
226 365 InterPro IPR011055 Duplicated hybrid motif
372 395 PRINTS PR00933 B-lytic metalloendopeptidase (M23) signature
372 395 InterPro IPR000841 Peptidase M23A, B-lytic metalloendopeptidase
293 311 PRINTS PR00933 B-lytic metalloendopeptidase (M23) signature
293 311 InterPro IPR000841 Peptidase M23A, B-lytic metalloendopeptidase
244 265 PRINTS PR00933 B-lytic metalloendopeptidase (M23) signature
244 265 InterPro IPR000841 Peptidase M23A, B-lytic metalloendopeptidase
266 284 PRINTS PR00933 B-lytic metalloendopeptidase (M23) signature
266 284 InterPro IPR000841 Peptidase M23A, B-lytic metalloendopeptidase
345 365 PRINTS PR00933 B-lytic metalloendopeptidase (M23) signature
345 365 InterPro IPR000841 Peptidase M23A, B-lytic metalloendopeptidase
32 418 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
27 31 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
1 31 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

2 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 3IT5
X-ray 2.00 Å A,B,E,G
43.5% 237-418
PDBe RCSB PDBj File
Viewing
PDB 3IT7
X-ray 2.14 Å A,B
43.5% 237-418
PDBe RCSB PDBj File
Loaded
AlphaFold PA1871
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.52

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 18.31 0.82
2 2.76 0.084
3 1.59 0.025

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

16 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

Show only:
Ligand Source crystal MW · LogP · TPSA Lipinski PAINS SMILES
TLA
3IT7 2.14 Å Open crystal
150.1 Da LogP -2.12 TPSA 115.1 ✓ Ro5 ✓ Clean [C@@H]([C@H](C(=O)O)O)(C(=O)O)O

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.