Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA1912
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: femI PA1912
Status: annotated
Amino acids: 168
Structure source: AlphaFold

GO

GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). GO:0015344 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: siderophore-iron(ferrioxamine)(out) + H+(out) = siderophore-iron(ferrioxamine)(in) + H+(in). GO:0016987 Sigma factors act as the promoter specificity subunit of eubacterial and plant plastid multisubunit RNA polymerases, whose core subunit composition is often described as alpha(2)-beta-beta-prime. Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, the sigma factor binds specifically to promoter elements. The sigma subunit is released once elongation begins. GO:0006352 The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0015891 The directed movement of siderophores, low molecular weight Fe(III)-chelating substances, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.867

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7

GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
GO:0015344 Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction: siderophore-iron(ferrioxamine)(out) + H+(out) = siderophore-iron(ferrioxamine)(in) + H+(in).
GO:0016987 Sigma factors act as the promoter specificity subunit of eubacterial and plant plastid multisubunit RNA polymerases, whose core subunit composition is often described as alpha(2)-beta-beta-prime. Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, the sigma factor binds specifically to promoter elements. The sigma subunit is released once elongation begins.
GO:0006352 The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
GO:0015891 The directed movement of siderophores, low molecular weight Fe(III)-chelating substances, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.

Sequence Features

Domain/signature hits from InterPro and related databases.

18 records

Show feature table

Start	End	DB	Term	Name
90	166	SUPERFAMILY	SSF88659	Sigma3 and sigma4 domains of RNA polymerase sigma factors
90	166	InterPro	IPR013324	RNA polymerase sigma factor, region 3/4-like
10	100	SUPERFAMILY	SSF88946	Sigma2 domain of RNA polymerase sigma factors
10	100	InterPro	IPR013325	RNA polymerase sigma factor, region 2
4	92	Gene3D	G3DSA:1.10.1740.10	-
4	92	FunFam	G3DSA:1.10.1740.10:FF:000009	RNA polymerase sigma factor
10	165	NCBIfam	TIGR02937	sigma-70 family RNA polymerase sigma factor
10	165	InterPro	IPR014284	RNA polymerase sigma-70 like domain
78	167	FunFam	G3DSA:1.10.10.10:FF:000427	RNA polymerase sigma factor
93	168	Gene3D	G3DSA:1.10.10.10	-
93	168	InterPro	IPR036388	Winged helix-like DNA-binding domain superfamily
9	166	PANTHER	PTHR43133	RNA POLYMERASE ECF-TYPE SIGMA FACTO
9	166	InterPro	IPR039425	RNA polymerase sigma-70 like
112	163	Pfam	PF08281	Sigma-70, region 4
112	163	InterPro	IPR013249	RNA polymerase sigma factor 70, region 4 type 2
112	163	CDD	cd06171	Sigma70_r4
15	80	Pfam	PF04542	Sigma-70 region 2
15	80	InterPro	IPR007627	RNA polymerase sigma-70 region 2

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

Loading 3D structure...

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA1912	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.867
3				0.639

PA1912