Protein profile

PA1992

sensor histidine kinase

Genome: NC_002516.2

Gene: ercS PA1992 Structure source: AlphaFold UniProt Q9I2B7
Export view
Amino acids 564
Annotations 8
Features 50
PDB binders 5
Druggability 0.873

Overview

Basic information about this protein and its source genome.

Accession
PA1992
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
ercS PA1992
Status
annotated
Amino acids
564
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.873
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0009927 Serves as a phospho-His intermediate enabling the transfer of phospho group between a hybrid kinase and a response regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Sequence Features

Domain/signature hits from InterPro and related databases.

50 records
Show feature table
Start End DB Term Name
28 48 Coils Coil Coil
311 422 SMART SM00387 HKATPase_4
311 422 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
30 155 SUPERFAMILY SSF55785 PYP-like sensor domain (PAS domain)
30 155 InterPro IPR035965 PAS domain superfamily
446 561 ProSiteProfiles PS50110 Response regulatory domain profile.
446 561 InterPro IPR001789 Signal transduction response regulator, receiver domain
202 267 SMART SM00388 HisKA_10
202 267 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
269 423 Gene3D G3DSA:3.30.565.10 -
269 423 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
44 102 Pfam PF13188 PAS domain
44 102 InterPro IPR000014 PAS domain
117 166 ProSiteProfiles PS50113 PAC domain profile.
117 166 InterPro IPR000700 PAS-associated, C-terminal
166 265 Gene3D G3DSA:1.10.287.130 -
40 164 NCBIfam TIGR00229 PAS domain S-box protein
40 164 InterPro IPR000014 PAS domain
269 423 FunFam G3DSA:3.30.565.10:FF:000049 Two-component sensor histidine kinase
55 156 CDD cd00130 PAS
55 156 InterPro IPR000014 PAS domain
39 155 Gene3D G3DSA:3.30.450.20 PAS domain
257 422 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
257 422 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
445 557 SMART SM00448 REC_2
445 557 InterPro IPR001789 Signal transduction response regulator, receiver domain
448 557 Pfam PF00072 Response regulator receiver domain
448 557 InterPro IPR001789 Signal transduction response regulator, receiver domain
39 155 FunFam G3DSA:3.30.450.20:FF:000184 Hybrid sensor histidine kinase/response regulator
347 361 PRINTS PR00344 Bacterial sensor protein C-terminal signature
347 361 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
382 400 PRINTS PR00344 Bacterial sensor protein C-terminal signature
382 400 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
406 419 PRINTS PR00344 Bacterial sensor protein C-terminal signature
406 419 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
312 420 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
312 420 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
185 266 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
185 266 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
29 560 PANTHER PTHR43047 TWO-COMPONENT HISTIDINE PROTEIN KINASE
157 202 Coils Coil Coil
200 263 CDD cd00082 HisKA
200 263 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
445 564 Gene3D G3DSA:3.40.50.2300 -
443 562 SUPERFAMILY SSF52172 CheY-like
443 562 InterPro IPR011006 CheY-like superfamily
448 547 CDD cd00156 REC
169 265 FunFam G3DSA:1.10.287.130:FF:000081 Hybrid sensor histidine kinase/response regulator
209 422 ProSiteProfiles PS50109 Histidine kinase domain profile.
209 422 InterPro IPR005467 Histidine kinase domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

3D visualization script Full viewer

Loading 3D structure...

Legend High Medium Low

Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA1992
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
4 0.873
3 0.365

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

55 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
6QRJ
Q9ABT2 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
BEF
5N2N
O22267 66.0 Da LogP 0.88 TPSA 0.0 ✓ Ro5 ✓ Clean [Be-](F)(F)F
EMC
3A0W
Q9X180 229.7 Da LogP 0.97 TPSA 0.0 ✓ Ro5 ✓ Clean CC[Hg+]
EMT
3A0W
Q9X180 382.8 Da LogP 2.91 TPSA 37.3 ✓ Ro5 ✓ Clean CC[Hg]Sc1ccccc1C(=O)O
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.