Protein profile

PA2278

arsenite-antimonite efflux pump ArsB

Genome: NC_002516.2

Gene: PA2278 arsB Structure source: AlphaFold UniProt Q9I1J6
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Amino acids 427
Annotations 7
Features 59
PDB binders 0
Druggability 0.936

Overview

Basic information about this protein and its source genome.

Accession
PA2278
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA2278 arsB
Status
annotated
Amino acids
427
Structure source
AlphaFold
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0042960 Enables the transfer of antimonite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters. GO:0008490 Enables the transfer of arsenite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters. GO:0015700 The directed movement of arsenite into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. GO:0046685 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an arsenic stimulus from compounds containing arsenic, including arsenates, arsenites, and arsenides. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.936
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0042960 Enables the transfer of antimonite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.
  • GO:0008490 Enables the transfer of arsenite from one side of a membrane to the other, up its concentration gradient. The transporter binds the solute and undergoes a series of conformational changes. Transport works equally well in either direction and is driven by a chemiosmotic source of energy. Secondary active transporters include symporters and antiporters.
  • GO:0015700 The directed movement of arsenite into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
  • GO:0046685 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an arsenic stimulus from compounds containing arsenic, including arsenates, arsenites, and arsenides.
  • GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
  • GO:0015105 Enables the transfer of arsenite from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

59 records
Show feature table
Start End DB Term Name
175 198 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
316 334 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
133 138 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
424 427 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
401 423 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
2 19 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
3 427 NCBIfam TIGR00935 arsenite/antimonite efflux transporter
3 427 InterPro IPR000802 Arsenical pump membrane protein, ArsB
1 1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
115 132 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 35 Phobius SIGNAL_PEPTIDE Signal peptide region
242 246 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
335 400 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
247 266 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
164 174 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
3 413 CDD cd01118 ArsB_permease
3 413 InterPro IPR000802 Arsenical pump membrane protein, ArsB
36 50 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
96 118 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
316 335 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
176 198 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
4 422 Pfam PF02040 Arsenical pump membrane protein
4 422 InterPro IPR000802 Arsenical pump membrane protein, ArsB
51 70 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
224 246 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
267 277 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
125 147 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
18 35 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
71 90 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
199 221 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
2 422 PANTHER PTHR43302 TRANSPORTER ARSB-RELATED
401 423 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
282 301 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
110 114 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
2 17 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
222 241 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
144 168 PRINTS PR00758 Arsenical pump membrane protein signature
144 168 InterPro IPR000802 Arsenical pump membrane protein, ArsB
364 389 PRINTS PR00758 Arsenical pump membrane protein signature
364 389 InterPro IPR000802 Arsenical pump membrane protein, ArsB
397 421 PRINTS PR00758 Arsenical pump membrane protein signature
397 421 InterPro IPR000802 Arsenical pump membrane protein, ArsB
101 125 PRINTS PR00758 Arsenical pump membrane protein signature
101 125 InterPro IPR000802 Arsenical pump membrane protein, ArsB
177 200 PRINTS PR00758 Arsenical pump membrane protein signature
177 200 InterPro IPR000802 Arsenical pump membrane protein, ArsB
278 302 PRINTS PR00758 Arsenical pump membrane protein signature
278 302 InterPro IPR000802 Arsenical pump membrane protein, ArsB
323 345 PRINTS PR00758 Arsenical pump membrane protein signature
323 345 InterPro IPR000802 Arsenical pump membrane protein, ArsB
217 242 PRINTS PR00758 Arsenical pump membrane protein signature
217 242 InterPro IPR000802 Arsenical pump membrane protein, ArsB
139 163 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
297 315 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
278 296 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
91 109 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
48 70 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
250 269 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
24 41 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2278
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
3 0.936
1 0.91
11 0.505