Protein profile

PA2426

extracytoplasmic-function sigma-70 factor

Genome: NC_002516.2

Gene: pvdS DT376_31030 GNQ48_20855 PAERUG_P19_London_7_VIM_2_05_10_05523 PA2426 CAZ10_34480 fecI_10 Structure source: AlphaFold UniProt G3XCV1 UniProt P95425
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Amino acids 187
Annotations 11
Features 17
PDB binders 0
Druggability 0.557

Overview

Basic information about this protein and its source genome.

Accession
PA2426
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
pvdS DT376_31030 GNQ48_20855 PAERUG_P19_London_7_VIM_2_05_10_05523 PA2426 CAZ10_34480 fecI_10
Status
annotated
Amino acids
187
Structure source
AlphaFold
GO
GO:0032993 A macromolecular complex containing both protein and DNA molecules. GO:0001216 A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets. GO:0016987 Sigma factors act as the promoter specificity subunit of eubacterial and plant plastid multisubunit RNA polymerases, whose core subunit composition is often described as alpha(2)-beta-beta-prime. Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, the sigma factor binds specifically to promoter elements. The sigma subunit is released once elongation begins. GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon. GO:0071281 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus. GO:0006352 The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.557
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

11 GO

Gene Ontology (GO)

11
  • GO:0032993 A macromolecular complex containing both protein and DNA molecules.
  • GO:0001216 A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets.
  • GO:0016987 Sigma factors act as the promoter specificity subunit of eubacterial and plant plastid multisubunit RNA polymerases, whose core subunit composition is often described as alpha(2)-beta-beta-prime. Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, the sigma factor binds specifically to promoter elements. The sigma subunit is released once elongation begins.
  • GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.
  • GO:0071281 Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus.
  • GO:0006352 The initial step of transcription, consisting of the assembly of the RNA polymerase preinitiation complex (PIC) at a gene promoter, as well as the formation of the first few bonds of the RNA transcript. Transcription initiation includes abortive initiation events, which occur when the first few nucleotides are repeatedly synthesized and then released, and ends when promoter clearance takes place.
  • GO:0045893 Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:1900378 Any process that activates or increases the frequency, rate or extent of secondary metabolite biosynthetic process.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.

Sequence Features

Domain/signature hits from InterPro and related databases.

17 records
Show feature table
Start End DB Term Name
18 169 NCBIfam TIGR02937 sigma-70 family RNA polymerase sigma factor
18 169 InterPro IPR014284 RNA polymerase sigma-70 like domain
20 83 Pfam PF04542 Sigma-70 region 2
20 83 InterPro IPR007627 RNA polymerase sigma-70 region 2
18 94 SUPERFAMILY SSF88946 Sigma2 domain of RNA polymerase sigma factors
18 94 InterPro IPR013325 RNA polymerase sigma factor, region 2
14 84 FunFam G3DSA:1.10.1740.10:FF:000008 RNA polymerase sigma factor PvdS
83 172 FunFam G3DSA:1.10.10.10:FF:000398 RNA polymerase factor sigma-70
82 172 Gene3D G3DSA:1.10.10.10 -
82 172 InterPro IPR036388 Winged helix-like DNA-binding domain superfamily
118 168 Pfam PF08281 Sigma-70, region 4
118 168 InterPro IPR013249 RNA polymerase sigma factor 70, region 4 type 2
7 180 PANTHER PTHR43133 RNA POLYMERASE ECF-TYPE SIGMA FACTO
7 180 InterPro IPR039425 RNA polymerase sigma-70 like
103 173 SUPERFAMILY SSF88659 Sigma3 and sigma4 domains of RNA polymerase sigma factors
103 173 InterPro IPR013324 RNA polymerase sigma factor, region 3/4-like
14 81 Gene3D G3DSA:1.10.1740.10 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2426
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.557
5 0.535