Protein profile

PA2473

glutathione S-transferase

Genome: NC_002516.2

Gene: PA2473 Structure source: Experimental + AlphaFold UniProt Q9I109
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Amino acids 214
Annotations 7
Features 24
PDB binders 3
Druggability 0.583

Overview

Basic information about this protein and its source genome.

Accession
PA2473
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA2473
Status
annotated
Amino acids
214
Structure source
Experimental + AlphaFold
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0004364 Catalysis of the reaction: RX + glutathione = an S-substituted glutathione + a halide anion + H+. GO:0016034 Catalysis of the reaction: 4-maleylacetoacetate = 4-fumarylacetoacetate. GO:0006749 The chemical reactions and pathways involving glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins; it has a specific role in the reduction of hydrogen peroxide (H2O2) and oxidized ascorbate, and it participates in the gamma-glutamyl cycle. GO:0006559 The chemical reactions and pathways resulting in the breakdown of L-phenylalanine. GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
47.458
Human E-value
4.05e-08
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
Unknown

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.583
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0004364 Catalysis of the reaction: RX + glutathione = an S-substituted glutathione + a halide anion + H+.
  • GO:0016034 Catalysis of the reaction: 4-maleylacetoacetate = 4-fumarylacetoacetate.
  • GO:0006749 The chemical reactions and pathways involving glutathione, the tripeptide glutamylcysteinylglycine, which acts as a coenzyme for some enzymes and as an antioxidant in the protection of sulfhydryl groups in enzymes and other proteins; it has a specific role in the reduction of hydrogen peroxide (H2O2) and oxidized ascorbate, and it participates in the gamma-glutamyl cycle.
  • GO:0006559 The chemical reactions and pathways resulting in the breakdown of L-phenylalanine.
  • GO:0003824 Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic.
  • GO:0009072 The chemical reactions and pathways involving aromatic amino acid family, amino acids with aromatic ring (phenylalanine, tyrosine, tryptophan).

Sequence Features

Domain/signature hits from InterPro and related databases.

24 records
Show feature table
Start End DB Term Name
86 213 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile.
86 213 InterPro IPR010987 Glutathione S-transferase, C-terminal-like
3 80 Pfam PF13417 Glutathione S-transferase, N-terminal domain
3 80 InterPro IPR004045 Glutathione S-transferase, N-terminal
1 81 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile.
1 81 InterPro IPR004045 Glutathione S-transferase, N-terminal
85 214 FunFam G3DSA:1.20.1050.10:FF:000017 Maleylacetoacetate isomerase
1 187 SFLD SFLDG00358 Main (cytGST)
1 85 FunFam G3DSA:3.40.30.10:FF:000119 Maleylacetoacetate isomerase
1 187 SFLD SFLDS00019 Glutathione Transferase (cytosolic)
1 187 InterPro IPR040079 Glutathione transferase family
87 208 CDD cd03191 GST_C_Zeta
87 208 InterPro IPR034330 Glutathione S-transferases, class Zeta , C-terminal
3 79 Gene3D G3DSA:3.40.30.10 Glutaredoxin
84 214 Gene3D G3DSA:1.20.1050.10 -
1 210 PANTHER PTHR42673 MALEYLACETOACETATE ISOMERASE
1 90 SUPERFAMILY SSF52833 Thioredoxin-like
1 90 InterPro IPR036249 Thioredoxin-like superfamily
81 211 SUPERFAMILY SSF47616 GST C-terminal domain-like
81 211 InterPro IPR036282 Glutathione S-transferase, C-terminal domain superfamily
1 74 CDD cd03042 GST_N_Zeta
1 74 InterPro IPR034333 Glutathione S-transferases, class Zeta , N-terminal
2 211 NCBIfam TIGR01262 maleylacetoacetate isomerase
2 211 InterPro IPR005955 Glutathione S-transferases, class Zeta

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 6JWK
X-ray 1.86 Å A,B
100.0% 1-214
PDBe RCSB PDBj File
Viewing
AlphaFold PA2473
AlphaFold — — full sequence — Loaded
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.583

Pockets (P2RANK)

Showing top-ranked P2Rank candidates by probability. Probability is color-coded per P2Rank calibration: high (≥ 0.5), medium (0.2 – 0.49), low (< 0.2).

P2RANK Sticks Spheres Surfaces Score Probability Labels Zoom Positions
1 3.0 0.098
2 2.35 0.061
3 1.55 0.023
4 0.57 0.0

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

53 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
GSH
4KDY
B8J605 307.3 Da LogP -2.21 TPSA 158.8 1 viol. ✓ Clean C(CC(=O)N[C@@H](CS)C(=O)NCC(=O)O)[C@@H](C(=O)O)N
MLA
4PX1
C5ATQ9 104.1 Da LogP -0.45 TPSA 74.6 ✓ Ro5 ✓ Clean C(C(=O)O)C(=O)O
TGG
4KAE
B8J605 423.4 Da LogP -2.47 TPSA 233.4 1 viol. ✓ Clean C(CC(=O)N[C@@H](CS[C@H](CC(=O)O)C(=O)O)C(=O)NCC…

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.