Protein profile

PA2480

two-component sensor

Genome: NC_002516.2

Gene: PA2480 Structure source: AlphaFold UniProt Q9I102
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Amino acids 440
Annotations 8
Features 39
PDB binders 2
Druggability 0.945

Overview

Basic information about this protein and its source genome.

Accession
PA2480
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA2480
Status
annotated
Amino acids
440
Structure source
AlphaFold
EC
2.7.13.3
GO
GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
N
Localization
CytoplasmicMembrane

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.945
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 7 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

7
  • GO:0005886 The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000155 Catalysis of the phosphorylation of a histidine residue in response to detection of an extracellular signal such as a chemical ligand or change in environment, to initiate a change in cell state or activity. The two-component sensor is a histidine kinase that autophosphorylates a histidine residue in its active site. The phosphate is then transferred to an aspartate residue in a downstream response regulator, to trigger a response.
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
  • GO:0007165 The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
  • GO:0016310 The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
  • GO:0016772 Catalysis of the transfer of a phosphorus-containing group from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

39 records
Show feature table
Start End DB Term Name
7 19 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide.
223 285 CDD cd00082 HisKA
223 285 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
334 440 SMART SM00387 HKATPase_4
334 440 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
401 419 PRINTS PR00344 Bacterial sensor protein C-terminal signature
401 419 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
371 385 PRINTS PR00344 Bacterial sensor protein C-terminal signature
371 385 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
424 437 PRINTS PR00344 Bacterial sensor protein C-terminal signature
424 437 InterPro IPR004358 Signal transduction histidine kinase-related protein, C-terminal
342 435 CDD cd00075 HATPase
172 440 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm.
1 439 PANTHER PTHR45436 SENSOR HISTIDINE KINASE YKOH
1 24 Phobius SIGNAL_PEPTIDE Signal peptide region
225 289 SMART SM00388 HisKA_10
225 289 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
148 171 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane.
1 6 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide.
232 440 ProSiteProfiles PS50109 Histidine kinase domain profile.
232 440 InterPro IPR005467 Histidine kinase domain
176 288 FunFam G3DSA:1.10.287.130:FF:000035 Two-component sensor histidine kinase
342 439 Pfam PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
342 439 InterPro IPR003594 Histidine kinase/HSP90-like ATPase
208 287 SUPERFAMILY SSF47384 Homodimeric domain of signal transducing histidine kinase
208 287 InterPro IPR036097 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily
227 288 Pfam PF00512 His Kinase A (phospho-acceptor) domain
227 288 InterPro IPR003661 Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
332 437 SUPERFAMILY SSF55874 ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase
332 437 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
296 440 Gene3D G3DSA:3.30.565.10 -
296 440 InterPro IPR036890 Histidine kinase/HSP90-like ATPase superfamily
20 24 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide.
16 147 Pfam PF08521 Two-component sensor kinase N-terminal
16 147 InterPro IPR013727 Two-component sensor kinase, N-terminal
25 147 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
5 27 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
147 169 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane.
176 288 Gene3D G3DSA:1.10.287.130 -

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

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Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2480
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.43
8 0.34

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

52 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
ANP
4BIW
P0AE82 506.2 Da LogP -2.06 TPSA 281.9 3 viol. ✓ Clean c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)…
PG0
5UKV
P71815 120.1 Da LogP -0.36 TPSA 38.7 ✓ Ro5 ✓ Clean COCCOCCO

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.