Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA2521
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA2521 czcB
Status: annotated
Amino acids: 484
Structure source: AlphaFold

GO

GO:0030313 An envelope that surrounds a bacterial cell and includes the cytoplasmic membrane and everything external, encompassing the periplasmic space, cell wall, and outer membrane if present. GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall. GO:0046914 Binding to a transition metal ions; a transition metal is an element whose atom has an incomplete d-subshell of extranuclear electrons, or which gives rise to a cation or cations with an incomplete d-subshell. Transition metals often have more than one valency state. Biologically relevant transition metals include vanadium, manganese, iron, copper, cobalt, nickel, molybdenum and silver. GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. GO:0060003 The directed movement of copper ions out of a cell or organelle.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.904

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

9 GO

Gene Ontology (GO)

9

GO:0030313 An envelope that surrounds a bacterial cell and includes the cytoplasmic membrane and everything external, encompassing the periplasmic space, cell wall, and outer membrane if present.
GO:0016020 A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
GO:0030288 The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin peptidoglycan layer (PGL), also referred to as a thin cell wall.
GO:0046914 Binding to a transition metal ions; a transition metal is an element whose atom has an incomplete d-subshell of extranuclear electrons, or which gives rise to a cation or cations with an incomplete d-subshell. Transition metals often have more than one valency state. Biologically relevant transition metals include vanadium, manganese, iron, copper, cobalt, nickel, molybdenum and silver.
GO:0022857 Enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other.
GO:0060003 The directed movement of copper ions out of a cell or organelle.
GO:0015679 The directed movement of copper ions across the plasma membrane.
GO:0046686 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cadmium (Cd) ion stimulus.
GO:0055085 The process in which a solute is transported across a lipid bilayer, from one side of a membrane to the other.

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records

Show feature table

Start	End	DB	Term	Name
186	321	Gene3D	G3DSA:2.40.50.100	-
16	20	Phobius	SIGNAL_PEPTIDE_C_REGION	C-terminal region of a signal peptide.
181	404	SUPERFAMILY	SSF111369	HlyD-like secretion proteins
322	403	Gene3D	G3DSA:2.40.30.170	-
220	285	Gene3D	G3DSA:1.10.287.470	Helix hairpin bin
404	481	FunFam	G3DSA:2.40.420.20:FF:000006	RND family efflux transporter MFP subunit
5	15	Phobius	SIGNAL_PEPTIDE_H_REGION	Hydrophobic region of a signal peptide.
319	405	FunFam	G3DSA:2.40.30.170:FF:000010	Efflux RND transporter periplasmic adaptor subunit
162	471	NCBIfam	TIGR01730	efflux RND transporter periplasmic adaptor subunit
162	471	InterPro	IPR006143	RND efflux pump, membrane fusion protein
404	483	Gene3D	G3DSA:2.40.420.20	-
5	27	TMHMM	TMhelix	Region of a membrane-bound protein predicted to be embedded in the membrane.
1	480	PANTHER	PTHR30097	CATION EFFLUX SYSTEM PROTEIN CUSB
1	4	Phobius	SIGNAL_PEPTIDE_N_REGION	N-terminal region of a signal peptide.
1	20	SignalP_EUK	SignalP-noTM	SignalP-noTM
168	399	Pfam	PF16576	Barrel-sandwich domain of CusB or HlyD membrane-fusion
168	399	InterPro	IPR032317	RND efflux pump, membrane fusion protein, barrel-sandwich domain
21	484	Phobius	NON_CYTOPLASMIC_DOMAIN	Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region.
266	286	Coils	Coil	Coil
1	20	Phobius	SIGNAL_PEPTIDE	Signal peptide region

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA2521	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
2				0.904
3				0.232

PA2521