Target Pathogen

Overview

Basic information about this protein and its source genome.

Accession: PA2586
Assembly: Pseudomonas aeruginosa PAO1, complete genome
Gene: PA2586 gacA
Status: annotated
Amino acids: 214
Structure source: AlphaFold

GO

GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons. GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon. GO:0035821 A process in which an organism effects a change in a biological process in another organism. GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. GO:1900233 Any process that activates or increases the frequency, rate or extent of single-species biofilm formation on inanimate substrate. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.

Target profile

Computed evidence for target prioritization.

Human off-target: No hit
Gut microbiome off-target: hit
Essential (DEG): Y
Localization: Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.701

Structure –

Pocket –

Sequence

Primary amino-acid sequence viewer.

MIKVLVVDDHDLVRTGITRMLADIEGLQVVGQADCGEDCLKLARELKPDVVLMDVKMPGIGGLEATRKLLRSQPDIKVVVVTVCEEDPFPTRLMQAGAAGYMTKGAGLEEMVQAIRQVFAGQRYISPQIAQQLALKSFQPQQHDSPFDSLSEREIQIALMIANCHKVQSISDKLCLSPKTVNTYRYRIFEKLSITSDVELALLAVRHGMVDAAS

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

7 GO

Gene Ontology (GO)

7

GO:0003700 A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.
GO:0035821 A process in which an organism effects a change in a biological process in another organism.
GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.
GO:1900233 Any process that activates or increases the frequency, rate or extent of single-species biofilm formation on inanimate substrate.
GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).

Sequence Features

Domain/signature hits from InterPro and related databases.

32 records

Show feature table

Start	End	DB	Term	Name
4	120	CDD	cd17535	REC_NarL-like
2	210	Gene3D	G3DSA:3.40.50.2300	-
2	208	PANTHER	PTHR43214	TWO-COMPONENT RESPONSE REGULATOR
2	208	InterPro	IPR039420	Transcriptional regulatory protein WalR-like
2	115	SMART	SM00448	REC_2
2	115	InterPro	IPR001789	Signal transduction response regulator, receiver domain
210	214	Coils	Coil	Coil
4	116	Pfam	PF00072	Response regulator receiver domain
4	116	InterPro	IPR001789	Signal transduction response regulator, receiver domain
164	180	PRINTS	PR00038	LuxR bacterial regulatory protein HTH signature
164	180	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
180	192	PRINTS	PR00038	LuxR bacterial regulatory protein HTH signature
180	192	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
150	164	PRINTS	PR00038	LuxR bacterial regulatory protein HTH signature
150	164	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
150	206	CDD	cd06170	LuxR_C_like
150	206	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
3	119	ProSiteProfiles	PS50110	Response regulatory domain profile.
3	119	InterPro	IPR001789	Signal transduction response regulator, receiver domain
1	132	SUPERFAMILY	SSF52172	CheY-like
1	132	InterPro	IPR011006	CheY-like superfamily
147	204	SMART	SM00421	luxrmega5
147	204	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
148	204	Pfam	PF00196	Bacterial regulatory proteins, luxR family
148	204	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
164	191	ProSitePatterns	PS00622	LuxR-type HTH domain signature.
164	191	InterPro	IPR000792	Transcription regulator LuxR, C-terminal
2	210	FunFam	G3DSA:3.40.50.2300:FF:000015	Two-component response regulator UvrY
132	211	SUPERFAMILY	SSF46894	C-terminal effector domain of the bipartite response regulators
132	211	InterPro	IPR016032	Signal transduction response regulator, C-terminal effector
143	208	ProSiteProfiles	PS50043	LuxR-type HTH domain profile.
143	208	InterPro	IPR000792	Transcription regulator LuxR, C-terminal

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry	Method	Resolution	Chain	Coverage	Links	Status
AlphaFold PA2586	AlphaFold	—	—	full sequence	—	Viewing

Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET	Sticks	Spheres	Surfaces	Druggability	Labels	Zoom	Positions
1				0.701

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

2 records

Highest-confidence structural evidence: ligands co-crystallized with this exact protein. If the source PDB is loaded in TPW, use Open crystal to inspect it in the structure viewer.

No PDB structure with a co-crystallized ligand found for this exact protein.

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Ligand	Source crystal	UniProt (homolog)	MW · LogP · TPSA	Lipinski	PAINS	SMILES
4QT	4ZMS	Q8DNC2	393.8 Da LogP 3.26 TPSA 84.9	✓ Ro5	✓ Clean	`c1(c(c(c(c(c1Br)OO)Br)OO)Br)N`
BEF	4LDZ	O34723	66.0 Da LogP 0.88 TPSA 0.0	✓ Ro5	✓ Clean	`[Be-](F)(F)F`

Experimental bioactivity from ChEMBL measured directly on this protein. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL bioactivity data found for this exact protein.

Bioactivity inferred from similar proteins in ChEMBL. Score = pchembl (−log Ki/IC₅₀; higher = more potent).

No ChEMBL hits found through similar proteins.

Proposed virtual-screening candidates from ZINC. Score = Tanimoto similarity to a known binder (0–1; higher = more similar).

No virtual-screening candidates for this protein.

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.

PA2586