Protein profile

PA2626

tRNA-specific 2-thiouridylase MnmA

Genome: NC_002516.2

Gene: PA2626 trmU mnmA Structure source: AlphaFold UniProt Q9I0L2
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Amino acids 375
Annotations 9
Features 20
PDB binders 0
Druggability 0.465

Overview

Basic information about this protein and its source genome.

Accession
PA2626
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
PA2626 trmU mnmA
Status
annotated
Amino acids
375
Structure source
AlphaFold
EC
2.8.1.13
GO
GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. GO:0000049 Binding to a transfer RNA. GO:0103016 Catalysis of the reaction: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein]. GO:0002143 The process in which a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps. GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.

Target profile

Computed evidence for target prioritization.

Human off-target
hit
Human identity (%)
56.452
Human E-value
3.5700000000000003e-43
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.465
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

1 EC 8 GO

Enzyme Commission (EC)

1

Gene Ontology (GO)

8
  • GO:0005737 The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
  • GO:0005524 Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
  • GO:0000049 Binding to a transfer RNA.
  • GO:0103016 Catalysis of the reaction: AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein].
  • GO:0002143 The process in which a uridine residue at position 34 in the anticodon of a tRNA is post-transcriptionally thiolated at the C2 position. This process involves transfer of a sulfur from cysteine to position C2 by several steps.
  • GO:0016740 Catalysis of the transfer of a group, e.g. a methyl group, glycosyl group, acyl group, phosphorus-containing, or other groups, from one compound (generally regarded as the donor) to another compound (generally regarded as the acceptor). Transferase is the systematic name for any enzyme of EC class 2.
  • GO:0008033 The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
  • GO:0016783 Catalysis of the transfer of sulfur atoms from one compound (donor) to another (acceptor).

Sequence Features

Domain/signature hits from InterPro and related databases.

20 records
Show feature table
Start End DB Term Name
2 216 Gene3D G3DSA:3.40.50.620 HUPs
2 216 InterPro IPR014729 Rossmann-like alpha/beta/alpha sandwich fold
11 248 SUPERFAMILY SSF52402 Adenine nucleotide alpha hydrolases-like
217 285 Gene3D G3DSA:2.30.30.280 -
217 285 InterPro IPR023382 tRNA-specific 2-thiouridylase MnmA-like, central domain superfamily
12 207 Pfam PF03054 tRNA methyl transferase HUP domain
12 363 CDD cd01998 tRNA_Me_trans
12 363 InterPro IPR004506 tRNA-specific 2-thiouridylase
289 363 Pfam PF20258 Aminomethyltransferase beta-barrel domain
289 363 InterPro IPR046885 tRNA-specific 2-thiouridylase MnmA-like, C-terminal domain
286 370 Gene3D G3DSA:2.40.30.10 Translation factors
286 369 FunFam G3DSA:2.40.30.10:FF:000023 tRNA-specific 2-thiouridylase MnmA
212 279 Pfam PF20259 tRNA methyl transferase PRC-barrel domain
212 279 InterPro IPR046884 tRNA-specific 2-thiouridylase MnmA-like, central domain
12 363 NCBIfam TIGR00420 tRNA 2-thiouridine(34) synthase MnmA
5 216 FunFam G3DSA:3.40.50.620:FF:000004 tRNA-specific 2-thiouridylase MnmA
217 284 FunFam G3DSA:2.30.30.280:FF:000001 tRNA-specific 2-thiouridylase MnmA
10 364 PANTHER PTHR11933 TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE
11 364 Hamap MF_00144 tRNA-specific 2-thiouridylase MnmA [mnmA].
11 364 InterPro IPR004506 tRNA-specific 2-thiouridylase

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

0 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
AlphaFold PA2626
AlphaFold — — full sequence — Viewing
Pocket details FPocket · P2Rank — toggle visibility and zoom from here, or open full viewer

Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
1 0.315
3 0.205