Protein profile

PA2657

two-component response regulator

Genome: NC_002516.2

Gene: carR PA2657 Structure source: Experimental + AlphaFold UniProt Q9I0I1
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Amino acids 223
Annotations 8
Features 27
PDB binders 1
Druggability 0.399

Overview

Basic information about this protein and its source genome.

Accession
PA2657
Assembly
Pseudomonas aeruginosa PAO1, complete genome
Gene
carR PA2657
Status
annotated
Amino acids
223
Structure source
Experimental + AlphaFold
GO
GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. GO:0032993 A macromolecular complex containing both protein and DNA molecules. GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response. GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon. GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription. GO:0051592 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus.

Target profile

Computed evidence for target prioritization.

Human off-target
No hit
Gut microbiome off-target
hit
Essential (DEG)
Y
Localization
Cytoplasmic

Selected Druggability evidence

Selected Druggability is the FPocket score chosen for ranking using the curated structure priority. The 3D viewer may show a different loaded structure, so its visible pockets can differ.

FPocket 0.399
Structure –
Pocket –

Sequence

Primary amino-acid sequence viewer.

Functional Annotations

Enzyme classification and Gene Ontology terms linked to this protein.

8 GO

Gene Ontology (GO)

8
  • GO:0005829 The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
  • GO:0032993 A macromolecular complex containing both protein and DNA molecules.
  • GO:0000156 Responds to a phosphorelay sensor to initiate a change in cell state or activity. The activity of the response regulator is regulated by transfer of a phosphate from a histidine residue in the sensor, to an aspartate residue in the response regulator. Many but not all response regulators act as transcriptional regulators to elicit a response.
  • GO:0000976 Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.
  • GO:0006355 Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
  • GO:0051592 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a calcium ion stimulus.
  • GO:0003677 Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
  • GO:0000160 A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins.

Sequence Features

Domain/signature hits from InterPro and related databases.

27 records
Show feature table
Start End DB Term Name
4 112 Pfam PF00072 Response regulator receiver domain
4 112 InterPro IPR001789 Signal transduction response regulator, receiver domain
133 216 CDD cd00383 trans_reg_C
133 216 InterPro IPR001867 OmpR/PhoB-type DNA-binding domain
1 80 Gene3D G3DSA:3.40.50.2300 -
118 219 Gene3D G3DSA:1.10.10.10 -
118 219 InterPro IPR036388 Winged helix-like DNA-binding domain superfamily
81 117 Gene3D G3DSA:6.10.250.690 -
1 80 FunFam G3DSA:3.40.50.2300:FF:000002 DNA-binding response regulator PhoP
1 118 SUPERFAMILY SSF52172 CheY-like
1 118 InterPro IPR011006 CheY-like superfamily
1 112 SMART SM00448 REC_2
1 112 InterPro IPR001789 Signal transduction response regulator, receiver domain
146 217 SMART SM00862 Trans_reg_C_3
146 217 InterPro IPR001867 OmpR/PhoB-type DNA-binding domain
123 217 SUPERFAMILY SSF46894 C-terminal effector domain of the bipartite response regulators
123 217 InterPro IPR016032 Signal transduction response regulator, C-terminal effector
147 216 Pfam PF00486 Transcriptional regulatory protein, C terminal
147 216 InterPro IPR001867 OmpR/PhoB-type DNA-binding domain
122 219 FunFam G3DSA:1.10.10.10:FF:000005 Two-component system response regulator
2 116 ProSiteProfiles PS50110 Response regulatory domain profile.
2 116 InterPro IPR001789 Signal transduction response regulator, receiver domain
3 119 CDD cd19934 REC_OmpR_EcPhoP-like
1 217 PANTHER PTHR48111 REGULATOR OF RPOS
1 217 InterPro IPR039420 Transcriptional regulatory protein WalR-like
125 219 ProSiteProfiles PS51755 OmpR/PhoB-type DNA-binding domain profile.
125 219 InterPro IPR001867 OmpR/PhoB-type DNA-binding domain

3D Structure

Selected loaded structure. Experimental PDB entries may cover only a portion of the sequence; predicted models typically cover the full protein.

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Structural evidence

1 + 1

Experimental PDB entries and predicted models. Click Switch to display a different structure in the viewer.

Entry Method Resolution Chain Coverage Links Status
PDB 8GC6
X-ray 1.30 Å A
55.2% 1-123
PDBe RCSB PDBj File
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AlphaFold PA2657
AlphaFold — — full sequence — Loaded
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Pockets (FPOCKET)

Showing top-ranked FPocket candidates by druggability. Druggability is color-coded: high (0.7 or higher), medium (0.4 to 0.69), low (below 0.4).

FPOCKET Sticks Spheres Surfaces Druggability Labels Zoom Positions
2 0.399
1 0.363
4 0.348

Ligand evidence

Ligands grouped by evidence source. PDB ligands keep the source crystal visible, and loaded crystals can be opened directly in the structure viewer.

1 records

Structural evidence inferred from similar proteins. The source crystal indicates where the ligand was observed; the UniProt column identifies the homologous protein carrying that ligand.

Show only:
Ligand Source crystal UniProt (homolog) MW · LogP · TPSA Lipinski PAINS SMILES
BEF
4S04
A0A0R4I965 66.0 Da LogP 0.88 TPSA 0.0 ✓ Ro5 ✓ Clean [Be-](F)(F)F

PDB and ChEMBL records on this protein are shown in full. ChEMBL records from similar proteins are capped at the top 100 per protein (by pchembl) and ZINC at the top 50 (Tanimoto ≥ 0.5). ADME columns are descriptor-based screening flags, not experimental toxicity results.